调控量子点对小鼠胚胎干细胞活性影响的研究

Experimental study on regulating the influence of quantum dots on the survival of murine embryo stem cells

目的:探讨调控量子点对小鼠胚胎干细胞活性的影响。方法:通过微波合成法制备荧光性能优越的量子点,并在体外对小鼠胚胎干细胞进行标记,采用激光共聚焦显微镜和流式细胞仪观察分析量子点对小鼠胚胎干细胞标记率、活力、增殖等方面的影响,并通过ROCK抑制剂Y-27632调控小鼠胚胎干细胞的活力和增殖能力。结果:用20 nmol.L-1的量子点标记小鼠胚胎干细胞培养24 h后,小鼠胚胎干细胞的标记率达到92.5%,存活率只有40.7%;使用10μmol.L-1Y-27632调控量子点标记的小鼠胚胎干细胞培养24 h后,小鼠胚胎干细胞的存活率提高20%。结论:量子点的浓度越大,小鼠胚胎干细胞的标记率越高,成活率越低,添加Y-27632可以阻止量子点标记的胚胎干细胞的凋亡,明显增加胚胎干细胞的生物活性。

Objective： To regulate the influence of quantum dots on the survival of murine embryo stem cells.Methods： We prepared the quantum dots by using microwave synthesis method;labeled the murine embryo stem cells in vitro with quantum dots;analyzed the influence of quantum dots on the murine embryo stem cells by using confocal microscopy and flow cytometer,such as labeling rate,viability and proliferation capability;regulated the viability and proliferation capability of murine embryo stem cells by adding ROCK inhibitor Y-27632.Results： Murine embryo stem cells had 92.5% labeled rate and 40.7% survival rate after cultured with 20 nmol·L-1 quantum dots for 24 hours,murine embryo stem cells had 20% survival rate of growth on the same culture condition after regulated by 10 μmol·L-1 Y-27632.Conclusion： The more concentration of quantum dots treated,the higher rate of murine embryo stem cells being labeled and the lower rate of murine embryo stem cells surviving.ROCK inhibitor Y-27632 could prevent murine embryo stem cells from apoptosis and increase murine embryo stem cells＇ bioactivity.