期刊文献+

荧光磁性纳米粒子体外标记骨髓间充质干细胞研究 被引量:5

Study of fluorescent supermagnetic nanoparticles labeled bone marrow mesenchymal stem cells in vitro
下载PDF
导出
摘要 目的:探索高效体外示踪骨髓间充质干细胞体系的方法。方法:采用贴壁培养法分离、纯化大鼠骨髓间充质干细胞,用流式细胞仪检测骨髓间充质干细胞CD29、CD90和CD45表达情况,用诱导剂诱导干细胞检测骨髓间充质干细胞的分化能力,采用普鲁士蓝染色,用激光共聚焦显微镜、磁共振成像仪观察荧光磁性纳米粒子标记的干细胞体外成像效果。结果:流式细胞仪检测CD29、CD90和CD45阳性表达率分别为84.69%、90.28%和0.72%;碱性磷酸酶染色和油红O染色结果均表明骨髓间充质干细胞成功分化为成骨细胞和脂肪细胞;普鲁士蓝染色、激光共聚焦显微镜成像和磁共振成像表明荧光磁性纳米粒子标记的骨髓间充质干细胞具有很好的成像效果。结论:荧光磁性纳米粒子作为示踪剂可以在体外标记骨髓间充质干细胞,为干细胞研究提供新思路。 Objective: To establish a system for efficiently tracking bone marrow mesenchymal stem cells(BMSCs) in vitro.Methods: BMSCs were harvested and purified from rats by adherent culture and then BMSCs expression of CD29,CD90 and CD45 were detected by using flow cytometry.To evaluate their ability to differentiate into multi-lineage cells,BMSCs were cultured in different induced media.Prussian blue staining,laser scanning confocal microscope,magnetic resonance imaging were used to observe labeled BMSCs in vitro after co-culturing with FMNPs.Results: FACS analysis showed that these cells were positive for markers CD29,CD90,CD45 as 84.69%,90.28% and 0.72%.ALP staining and oil red O staining indicated that BMSCs were inducted into osteoblasts and adipose cells.Prussian blue staining,laser scanning confocal and magnetic resonance imaging showed that the prepared FMNPs labeled BMSCs had high quality images.Conclusion: As an important stem cell tracer,FMNPs could label BMSCs and provided a new method for stem cell research.
出处 《东南大学学报(医学版)》 CAS 2011年第1期43-47,共5页 Journal of Southeast University(Medical Science Edition)
基金 国家重点基础研究发展计划(973计划)项目(2010CB933901)
关键词 荧光磁性纳米粒子 骨髓间充质干细胞 磁共振成像 示踪剂 fluorescent supermagnetic nanoparticles mesenchymal stem cells magnetic resonance imaging tracer
  • 相关文献

参考文献10

  • 1BIANCO P,RIMINUCCI M,GRONTHOS S,et al.Bone marrow stromal stem cells:nature,biology,and potential applications[J].Stem Cells,2001,19(3):180-192.
  • 2JI J,RUAN J,CUI D.Advance of nanotechnology in the stem cells research and development[J].Nano Biomed Eng,2010,2(1):67-90.
  • 3WANG Z,RUAN J,CUI D.Advances and prospect of nanotechnology in stem cells[J].Nanoscale Res Lett,2009,4(7):593-605.
  • 4ZHU H,GUO Z K,JIANG X X,et al.A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone[J].Nature Protocols,2010,5(3):550-560.
  • 5HE R,YOU X G,SHAO J,et al.Core/shell fluorescent magnetic silica-coated composite nanoparticles for bioconjugation[J].Nanotechnology,2007,18:1-7.
  • 6JIANG Y,JAHAGIRDAR B N,REINHARDT R L,et al.Pluripotency of mesenchymal stem cells derived from adult marrow[J].Nature,2002,418:41-49.
  • 7OSWALD J,BOXBERGER S,JORGENSEN B,et al.Mesenchymal stem cells can be differentiated into endothelial cells in vitro[J].Stem Cells,2004,22:77-384.
  • 8HSIAO J K,TAI M F,CHU H H,et al.Magnetic nanoparticle labeling of mesenchymal stem cells without transfection agent:cellular behavior and capability of detection with clinical 1.5 T magnetic resonance at the single cell level[J].Magn Reson Med,2007,58:717-724.
  • 9CUI D,LI Z.Fluorescent magnetic nanoparticles for in vivo targeted imaging and hyperthermia therapy of prostate cancer[J].Nano Biomed Eng,2009,1(1):61-74.
  • 10RICKS D M,KUTNER R,ZHANG X Y,et al.Optimized lentiviral transduction of mouse bone marrow-derived mesenchymal stem cells[J].Stem Cells Dev,2008,17:441-450.

同被引文献52

引证文献5

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部