壳聚糖修饰单壁碳纳米管的制备与细胞毒性研究

Preparation and cell cytotoxicity of CHI/SWCNT-COOHs

目的:制备稳定的水溶性的壳聚糖/碳纳米管(CHI/SWCNT-COOHs)纳米材料,对其表征并检测纳米材料的细胞毒性。方法:市售单壁碳纳米管(Raw SWCNTs)以强酸处理,形成残缺的羧基化单壁碳纳米管(SWCNT-COOHs),然后,低分子壳聚糖(CHI)与SWCNT-COOHs反应制备壳聚糖/碳纳米管(CHI/SWCNT-COOHs)纳米材料;运用透射电镜技术、拉曼光谱技术检测纳米材料的性状;通过台盼蓝染色计算纳米材料对A549细胞存活率的影响,计算纳米材料营养耗竭后对单细胞克隆的影响;应用扫描电镜检测其对红细胞形态的影响。结果:CHI/SWCNT-COOHs在水系中有很好的稳定性,对细胞无明显毒性,营养耗竭量减少,对红细胞形态没有显著影响。结论:CHI/SWCNT-COOHs性状稳定,具有纳米特性,细胞毒性低。

Objective：To synthesis SWCNT-based CHI/SWCNT-COOHs nano-materials and evaluate its in vitro cell toxicity.Methods：Raw single walled carbon nanotubes（SWCNTs） were treated by acid oxidation to form carboxylic SWCNT-COOHs.Then,SWCNT-COOHs were employed as native carriers wrapped with low molecular weight chitosan（CHI） through electrostatic force.The CHI/SWCNT-COOHs samples were charactered by using transmission electron microscopy（TEM） and Raman spectroscopy.A549 cell viability was determined by trypan blue dye exclusion assay after being treated with nano-materials.Statistics of the single cell colonies was obtained by nutritional depletion associated with the treatment of CNTs for one week.Scanning electron microscopy was used to observe the morphology structural of the red blood cell after treating with CNTs to them.Results：With high water stability,CHI/ SWCNT-COOHs could greatly improve the survival rate of cells,reduce nutrient depletion,and cause no significant change in red blood cell morphology.Conclusion：CHI/SWCNT-COOHs have good stability in water,with nano-features and low cytotoxicity.