祛风通络方对阿霉素肾病大鼠Pododcin mRNA表达及足细胞足突形态的影响

Effects of Qufengtongluo Recipe on podocin mRNA expression and podocyte morphology in rats with adriamycin-induced nephropathy

目的观察祛风通络方对阿霉素肾病大鼠Pododcin mRNA表达及足细胞足突形态的影响,探讨祛风通络方治疗肾病蛋白尿的分子生物学机制。方法 SD大鼠随机分为空白组和造模组,以阿霉素(6 mg/kg)尾静脉注射法造模,模型成功后将大鼠随机分为模型组、祛风组、激素组和苯那组,并给予相应干预。用RT-PCR法测定肾组织Podocin mRNA表达,透射电镜观察足细胞及足突形态变化。结果 (1)模型成功与空白组比较,造模组大鼠Podocin mRNA表达明显下调(P<0.01),足细胞足突融合甚至消失,足细胞有脱落、细胞数明显减少。(2)干预结束后与模型组比较,各干预组PodocinmRNA表达均上调(P<0.01);祛风组足细胞及足突形态改善明显。结论祛风通络方可能通过上调阿霉素肾病大鼠肾小球足细胞Podocin mRNA表达,改善足细胞及足突形态结构而达到防治蛋白尿的目的。

Objective To observe the effects of Qufengtongluo Recipe（QFTLR） on the expression of podocin mRNA and podocyte morphology in rats with adriamycin-induced nephropathy（AN）,and explore the possible mechanism mediating the therapeutic effect of QFTLR on nephropathic proteinuria.Methods SD rats were randomized into normal control group,AN model group（established by a single injection of adriamycin via the tail vein）,and 3 intervention groups with QFTLR,prednisone,or benazepril treatment.After the corresponding treatments,the expression of podocin mRNA in the renal tissues was detected by RT-PCR methods,and the morphological changes of the podocytes were examined by electron microscope.Results Compared with the normal control group,the AN model group showed significantly lowered expressions of podocin mRNA（P0.01） with reduced podocytes and widening,fusion or even absence of the foot processes（FP）.Intervention with QFTLR significantly increased the expression of podocin mRNA（P0.01） and the number of podocytes,and obviously lessened the structural changes of the FP.Conclusion QFTLR can produce therapeutic effect against nephropathic proteinuria possibly by up-regulating the expression of podocin mRNA and improving the morphological changes of the podocytes.