Wistar大鼠C6胶质瘤脑移植模型的应用

Establishment of a Wistar rat model bearing brain glioma C6 cells and its experimental application

目的通过观察C6胶质瘤大鼠脑移植模型,了解该模型的生物学特点,以帮助实验中有效应用。方法 Wistar大鼠20只,将活性较好的C6胶质瘤细胞微量移植入大鼠大脑半球,观察肿瘤生长特点及行为学改变。结果肿瘤移植后第3天,TW2即可出现轻微高信号改变。15/18的大鼠在第5天至少能看到2个层面的信号改变。16/18的大鼠在第11天能看见实质性肿瘤。13/18的大鼠第14天生长至半侧大脑的1/2。14/18的大鼠第20天大部分肿瘤达到半侧大脑的2/3,部分生长至对侧半球。结论实验可能的最佳时间窗在14～18 d。同时,肿瘤移植的初始细胞浓度、细胞活性和移植部位可能也对实验时间窗会有较大影响。

Objective To establish a rat model bearing brain glioma and investigate the optimal conditions for its experimental application.Methods？ C6 cells were implanted into the unilateral brain hemisphere of 20 Wistar rats.The growth behaviors of the brain tumor and behavioral changes of the rats were observed at different time points after the implantation.Results On day 3 after the implantation,only a slight increase of signal intensity was observed on T2-weighted images.By day 5,the tumor became visible in 15/18 of the rats in at least two sections.By day 11,16/18 of the rats showed space-occupying effect in the brain,and by day 14,the tumor occupied over 1/2 of the hemisphere in 14/18 of the rats.By day 20,14/18 of the rats showed a tumor mass occupying over 2/3 of the hemisphere,and some tumor cells had migrated into the contralateral hemisphere.Conclusion In this model of brain glioma,the optimal time widow for experiment is between 14 and 18 days after the cell implantation.The cell density and viability for implantation and the site of implantation may also affect the experimental time widow.