脑出血急性期内皮祖细胞血管内皮生长因子mRNA及蛋白的表达和分泌增加

Expression of Vascular endothelial growth factor mRNA and protein in endothelial progenitor cells in patients during acute cerebral hemorrhage associated with hypertension

目的 探讨高血压脑出血急性期患者外周血来源的内皮祖细胞（EPCs）血管内皮生长因子（VEGF）在转录、蛋白合成,以及培养上清液和血清中含量的变化.方法 分离、诱导分化高血压脑出血急性期患者和高血压患者各16例外周血EPCs,逆转录PCR方法测定EPCs VEGF mRNA表达水平,Western印迹方法检测VEGF蛋白表达水平;测定EPCs培养上清液和血清VEGF含量,并进行比较.结果 高血压脑出血组和高血压组相比,EPCs VEGF mRNA（0.186±0.035,0.090±0.031,t=8.318）,蛋白表达（0.223±0.028,0.169±0.022,t=3.744）,培养上清液VEGF含量[（414±37）、（316±29）pg/ml,t=8.270]和血清VEGF含量[（408±49）、（222±34）pg/ml,t=12.406]均明显增高,差异具有统计学意义（均P＜0.01）.结论 脑出血急性期高血压患者EPCs VEGF的mRNA和蛋白质表达上调,EPCs和机体分泌VEGF增加.

Objective To explore the expression of vascular endothelial growth factor （VEGF）mRNA and protein in endothelia progenitor cells （EPCs） and VEGF in the culture medium and serum of patients during acute period of cerebral hemorrhage associated with hypertension （APCHH）. Methods Mononuclear cells from peripheral blood of patients with APCHH （ 16 patients） and hypertension （ 16 patients） were isolated and induced to EPCs. Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） analysis was performed to assay VEGF mRNA. VEGF protein was assessed by Western blotting. The VEGF protein level in patient＇s serum and culture medium （ at day 7 ） were assayed using VEGF ELISA Kit and compared between APCHH group and hypertension group. Results Compared with hypertension group, VEGF mRNA （0. 186 ±0. 035 versus 0.090 ±0.031, t =8.318, P 〈0.0l ） and protein （0. 223 ± 0. 028 versus 0.169 ± 0. 022, t = 3. 744, P 〈 0. 01 ） expression of EPCs, the concentration of VEGF protein in the supernatant （414 ±37 versus 316 ±29, t =8. 270, P 〈0. 01 ） and in serum （408 ±49versus 222 ±34, t = 12.406, P 〈0. 01 ） were all significantly increased in APCHH group. Conclusion The VEGF protein levels in serum of patients and in the culture medium, VEGF mRNA and protein expression in EPCs were all significantly increased during acute periods of cerebral hemorrhage.