尾加压素Ⅱ对人晶状体上皮细胞一氧化氮合成的影响

Effect of urotensin Ⅱ on synthesis of nitric oxide in human lens epithelial cells

目的探讨新型生物活性物质尾加压素Ⅱ(UrotensinⅡ,U-Ⅱ)对人晶状体上皮细胞(human lens epithelial cells,HLEC)内一氧化氮合酶(nitric oxide sythase,NOS)活性及一氧化氮(nitric oxide,NO)合成的影响。方法用不同浓度的U-Ⅱ(1×10-9mol.L-1、10×10-9mol.L-1、100×10-9mol.L-1)干扰体外培养的HLEC,采用化学比色法和硝酸还原酶法测定HLEC内NOS活性和NO含量。结果空白对照组、(1×10-9mol.L-1、10×10-9mol.L-1、100×10-9mol.L-1)U-Ⅱ组的NOS活力值分别为(3.407±0.168)U.mg-1、(4.058±0.169)U.mg-1、(4.921±0.298)U.mg-1和(5.598±0.897)U.mg-1;NO含量分别为(0.747±0.183)μmol.g-1、(1.238±0.232)μmol.g-1、(1.531±0.101)μmol.g-1、(1.850±0.114)μmol.g-1。与空白对照组相比,U-Ⅱ呈浓度依赖性增强细胞内的NOS活性,刺激NO合成,差异均有显著统计学意义(均为P<0.01)。结论 U-Ⅱ可使HLEC内NOS活性增强和NO生成增多,提示U-Ⅱ可能通过NOS/NO途径促进HLEC增殖。

Objective To investigate the effect of new bioactive substance urotensin Ⅱ（U-Ⅱ）on activity of nitric oxide synthase（NOS）and synthesis of nitric oxide（NO）in human lens epithelial cells（HLEC）.Methods The HLEC cultured in vitro were interfered with different concentrations of U-Ⅱ（1×10-9 mol·L-1,10×10-9 mol·L-1,100×10-9 mol·L-1）,the NOS activity and NO level in HLEC were detected by chemical colorimetry and nitric acid reductase method,respectively.Results The NOS activity were（3.407±0.168）U·mg-1,（4.058±0.169）U·mg-1,（4.921±0.298）U·mg-1 and（5.598±0.897）U·mg-1 in control group,10-9 mol·L-1,10×10-9 mol·L-1,100×10-9 mol·L-1 U-Ⅱ groups,and the NO level were（0.747±0.183）μmol·g-1,（1.238±0.232）μmol·g-1,（1.531±0.101）μmol·g-1 and（1.850±0.114）μmol·g-1,respectively.Compared with control group,U-Ⅱ enhanced the NOS activity and NO synthesis obviously in a dose-dependent manner,and there were significant differences（all P0.01）.Conclusion U-Ⅱ enhances the NOS activity and NO synthesis,which shows U-Ⅱ may promote the proliferation of HLEC through NOS/NO pathway.