不同浓度葡萄糖、胰岛素对新生大鼠心肌细胞脂联素表达的影响

Influence of glucose and insulin on expression of adiponection in neonatal rat cardiomyocytes

目的:观察心肌细胞能否表达脂联素(APN),并探讨高糖、高胰岛素状态下心肌细胞APN表达的变化。方法:Sprague-Dawley(SD)大鼠的乳鼠心肌细胞原代培养,分为葡萄糖6组:高渗对照组、5.5 mmol/L组、20 mmol/L组、25 mmol/L组、30 mmol/L组和50 mmol/L组。胰岛素6组:0 mol/L组、10-10 mol/L组、10-9 mol/L组、10-8 mol/L组、10-7 mol/L组和10-6 mol/L组。干预48 h,用RT-PCR法测定心肌细胞APNmRNA表达的差异,并检测心肌细胞APN蛋白表达的变化。结果:在葡萄糖浓度小于30 mmol/L组中,心肌细胞APN表达随葡萄糖浓度的增加而减低(P<0.05),30 mmol/L和50 mmol/L组APN表达显著差异;渗透压对于心肌细胞APN表达无影响;在胰岛素浓度低于10-7 mol/L组中,心肌细胞APN表达随胰岛素浓度的增加而减低(P<0.05),在高于10-7 mol/L组中,心肌APN表达无显著差异。结论:心肌细胞能够表达脂联素。在一定范围内,随葡萄糖、胰岛素浓度增加,心肌细胞APN的表达水平下降。

AIM： To study the influence of glucose or insulin at different concentrations on the expression of adiponectin in cardiomyocytes. METHODS： Myocardial cells were isolated from the hearts of neonatal Sprague - Dawley rais and primarily cultured. The cells stimulated with glucose at concentrations of 5.5, 20, 25, 30, and 50 mmol/L re- spectively were divided into 5 groups and a hypertonic control group was set up. The cells treated with insulin at concentrations of 0, 10 - 10, 10 -9, 10 -s, 10-7 and 10 -6 mol/L were also divided into 6 groups. The mRNA and protein levels of adiponectin（APN） were determined by RT - PCR and Western blotting 48 h after the intervention with glucose or insulin. RESULTS： （ 1 ） The expression of adiponectin decreased with the elevation of glucose concentration at the doses lower than 30 mmol/L（P 〈0. 05）. When higher than 30 mmol/L, the expression of adiponectin didn＇t change significantly. The osmotic pressure did not affect the adiponectin expression in cardiomyocytes. （2） The expression of adiponectin decreased with the elevation of insulin concentration at the doses lower than 10-7 mmol/L（P 〈 0. 05 ）. When higher than 10-7 mmol/ L, the expression of adiponectin didn＇t change significantly. CONCLUSION ： The expression of APN decreases with the elevation of glucose or insulin concentrations within a certain range of dosage.