摘要
目的:研究口虾蛄提取物(EOS)对人低分化鼻咽癌细胞系(CNE-2Z)中P53、环氧化酶2(COX-2)和血管内皮生长因子(VEGF)表达的影响及意义。方法:不同浓度EOS(0 mg/L、125 mg/L、250 mg/L和500 mg/L)作用CNE-2Z细胞24 h后,Western blotting检测P53蛋白表达,免疫细胞化学法及RT-PCR法分别检测COX-2、VEGF蛋白和mRNA的表达。结果:CNE-2Z细胞中P53蛋白、COX-2和VEGF蛋白和mRNA表达均随EOS作用浓度增高而逐渐降低,呈量效关系(P<0.01);且COX-2和VEGF表达量呈正相关(P<0.05),COX-2和P53表达量亦呈正相关(P<0.05)。结论:EOS可能通过抑制CNE-2Z细胞中P53蛋白的表达,干扰COX-2和VEGF的表达而发挥其抗肿瘤作用的。
AIM: To study the inhibitory effect of the extract of Oratosquilla(EOS) on the expression of P53, cyclooxygenase -2 (COX -2) and vascular endothelial growth factor(VEGF) in nasopharyngeal carcinoma cell line CNE - 2Z. METHODS: CNE - 2Z cells were treated with different concentrations of EOS for 24 h. The methods of RT - PCR and immunocytochemistry were used to detect the expression of COX - 2 and VEGF at mRNA and protein levels, respectively. The protein expression of P53 was determined by Western blotting. RESULTS: After treated with EOS, the protein expression of 1)53 in CNE -2Z cells was decreased(P 〈0. 01 ), and the expression of COX -2 and VEGF at mRNA and protein levels was also significantly decreased in a dose - dependent manner(P 〈0. 01 ). The expression of COX -2 was positively correlated with that of VEGF(P 〈0. 05 ), and a positive correlation between the expression of COX- 2 and P53 protein(P 〈 0. 05) was also observed. CONCLUSION: EOS may play its antitumor effect by inhibiting the expression of P53, COX -2 and VEGF in nasopharyngeal carcinoma cell line CNE -2Z.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第2期390-393,共4页
Chinese Journal of Pathophysiology
基金
湛江市科技攻关资助项目(No.2009C3101011)
广东医学院青年基金资助项目(No.XQ0502)
关键词
口虾蛄
鼻咽癌
蛋白质P53
环氧化酶2
血管内皮生长因子
Oratosquilla oratoria
Nasopharyngeal neoplasms
Protein P53
Cyclooxygenase 2
Vascular endothelial growth factors