口虾蛄提取物对人鼻咽癌细胞P53、COX-2和VEGF表达的影响

Effects of extract of Oratosquilla on expression of P53,COX-2 and VEGF in human nasopharyngeal carcinoma cell line

目的:研究口虾蛄提取物(EOS)对人低分化鼻咽癌细胞系(CNE-2Z)中P53、环氧化酶2(COX-2)和血管内皮生长因子(VEGF)表达的影响及意义。方法:不同浓度EOS(0 mg/L、125 mg/L、250 mg/L和500 mg/L)作用CNE-2Z细胞24 h后,Western blotting检测P53蛋白表达,免疫细胞化学法及RT-PCR法分别检测COX-2、VEGF蛋白和mRNA的表达。结果:CNE-2Z细胞中P53蛋白、COX-2和VEGF蛋白和mRNA表达均随EOS作用浓度增高而逐渐降低,呈量效关系(P<0.01);且COX-2和VEGF表达量呈正相关(P<0.05),COX-2和P53表达量亦呈正相关(P<0.05)。结论:EOS可能通过抑制CNE-2Z细胞中P53蛋白的表达,干扰COX-2和VEGF的表达而发挥其抗肿瘤作用的。

AIM： To study the inhibitory effect of the extract of Oratosquilla（EOS） on the expression of P53, cyclooxygenase -2 （COX -2） and vascular endothelial growth factor（VEGF） in nasopharyngeal carcinoma cell line CNE - 2Z. METHODS： CNE - 2Z cells were treated with different concentrations of EOS for 24 h. The methods of RT - PCR and immunocytochemistry were used to detect the expression of COX - 2 and VEGF at mRNA and protein levels, respectively. The protein expression of P53 was determined by Western blotting. RESULTS： After treated with EOS, the protein expression of 1）53 in CNE -2Z cells was decreased（P 〈0. 01 ）, and the expression of COX -2 and VEGF at mRNA and protein levels was also significantly decreased in a dose - dependent manner（P 〈0. 01 ）. The expression of COX -2 was positively correlated with that of VEGF（P 〈0. 05 ）, and a positive correlation between the expression of COX- 2 and P53 protein（P 〈 0. 05） was also observed. CONCLUSION： EOS may play its antitumor effect by inhibiting the expression of P53, COX -2 and VEGF in nasopharyngeal carcinoma cell line CNE -2Z.