TRPC6介导DU145细胞条件培养液诱导人脐静脉内皮细胞增殖的研究

TRPC6 mediates the proliferation of human umbilical vein endothelial cells induced by DU145 conditioned medium

目的探讨瞬时受体势C6（TRPC6，transient receptor potential canonical6）在人前列腺癌细胞（DU145）的培养上清液诱导人脐静脉血管内皮细胞（hUVEC）增殖中的作用。方法以DU145细胞培养上清液和DMEM培养基的不同比例混合液培养的hUVEC细胞。MTF法检测细胞增殖情况的变化；PCR和Western-blot法分别检测TRPC6基因和蛋白的表达情况。结果hUVEC经前列腺癌细胞培养上清液作用48h后，细胞增殖明显增加，TRPC6基因和蛋白的表达上调。结论前列腺癌细胞培养上清液能明显促进人脐静脉内皮细胞的增殖，其机制可能是通过上调TRPC6来介导。

Objective To investigate the influence of transient receptor potential canonical 6 （TR- PC6） on the proliferation of hUVEC induced by the supernatant from human prostate cancer cells （DU145）. Methods Experiment group was treated with the mixture of DU145 cells supernatant and DMEM medium in different ratio, control group was treated with DMEM medium. The change of cell proliferation was detected by MTT method, and TRPC6 gene and protein was detected by PCR and Western-blot methods respectively. Results After incubated for 48h with the supernatant from cultured DUI45 cells, the population of hUVECs increased obviously, and the expression of TRPC6 genes and protein had clearly up-regulation. Conclusion The supernatant from cultured DU145 cells could effectively enhance the proliferation of hUVECs. The mechanisms might be that the proliferation of human umbilical vein endothelial cells was mediated by TRPC6.