Muc-1基因mRNA在肺腺癌过表达的临床意义

The associated investigation between overexpressed MUC-lmRNA and the pathogenesis of lung adenocarcinoma

目的探讨MUC-1基因的mRNA在肺腺癌过表达的临床意义。方法收集23例配对液氮保存的新鲜肺腺癌组织与其配对的正常肺组织，Trizol方法常规提取这些组织总RNA。在将这些总RNA逆转录成cDNA后，利用实时荧光定量PCR检测MUC-1基因在两种组织中的表达情况。分析MUC-1表达变化与肺腺癌发病之间的关系。结果与相应自身23个正常肺组织相比，MUC-1基因在20个（87％）肺腺癌组织中明显高表达。MUC-1基因在晚期肺腺癌中的表达比早中期肺腺癌中要高（平均2．2倍）。有淋巴结和远处转移肺腺癌中，MUC-1基因要比没有发生转移的肺腺癌组织中表达相应增高（平均2．5倍）。结论MUC-1在肺腺癌中高表达可能参与了肺腺癌的发生发展。

Objective To explore the preliminary significance of overexpressed MUC-1 mRNA in the pathogenesis of lung adenocarcinoma. Method Total RNAs of 23 paired fresh lung samples and lung adenocarcinoma samples were extracted by routine Trizol way. After total RNAs were transcribed into cD- NA, the expression of MUC-1 gene was detected in these samples by real-time PCR. Finally, the correlation of MUC-1 expression with the pathogenesis of lung adenocarcinoma was analyzed. Result Compared with normal lung tissues, the expression of MUC-1 in 20（87% ） lung ad samples was markedly increased. Compared with early and mid-stage lung adenoearcinoma samples, the overexpressed MUC-1 in late stage lung adenocarcinoma samples was increased by 2.2 folds. In addition, compared with primary lung adenocarcinoma samples without lymph node metastasis, MUC-1 mRNA was increased in primary lung adenocarcinoma samples with lymph node and distant metastasis （2.5 folds）. Conclusion Overexpressed MUC-1 may participate in the pathogenesis of lung adenocarcinoma.