鲮、麦鲮和野鲮之RAPD的遗传标记

THE GENETIC MARKERS OF CIRRHINA MOLITORELLA,C.MRIGOLA AND LABEO ROHIT FROM RAPD

以野生和池塘养殖的64个凌（Cirrhinamonlitorella）、10个麦坡（C．mrigola）和10个野峡（Labeorohita）样本为材料进行了随机扩增多态性DNA（RAPD）遗传分析。通过26个10bp随机引物扩增鲮、麦峻和野峻样品分别得到107、73、87个DNA片断，其多态位点百分率分别为35．5％，39．7％，37．9％。鲮与野鲮，麦鲮与野鲮及鲮与麦鲮的遗传距离分别为0．22，0．58，0．64。利用UPAMA和NJ建立了3种鲮之间分子水平上的亲缘关系图，认为鲮与野鲮亲缘关系比麦鲮与野鲮和鲮与麦鲮更近，与同功酶相近，与传统分类不一致。

The genetic assessment of 64 wild and cultivated mud carp, Cirrhina molitorella, 10 India mrigal, C. mrigola and 10 India rohu, Label rohita is inferred from its RAPD analysis. 107, 73, 87 DNA fragments in mud carp, India mrigal and India rohu are amplified respectively by 26 10bp-primers. The polymorphic loci percentage is 35. 5%,39. 7%, 37. 9% respectively. The genetic distance of mud carp-India rohu, mud carp-India mrigal and India mrigal-India rohu is 0. 22, 0. 58, 0. 64 respectively. The relationship of mud carp-India rohu is more close than mud carp-India mrigal and India mrigal-India rohu by the dendrogram of the three species based on UPGMA and NJ. This is similar to allozyme analysis, but there is differential from traditional taxonomy.