克氏原螯虾卵黄发生过程中卵巢和大颚器孕酮含量的变化

CHANGES OF PROGESTERONE CONTENTS OF OVARY AND MANDIBULAR ORGAN IN PROCAMBARUS CLARKII DURING THE VITELLOGENESIS

运用放射免疫测定法，研究克氏原螫虾卵黄发生过程中卵巢和大颚器的孕酮含量变化。其中次级卵黄发生早期的卵巢孕酮含量最高，卵黄发生前期卵巢孕酮含量次之，次级卵黄发生中期的卵巢孕酮含量显著降低，产后期卵巢检测不出孕酮含量。表明随着卵巢的成熟和产卵，卵巢中的孕酮含量逐渐下降，推测孕酮与卵黄发生的启动有关。而在不同发育时期的大颚器中均未能测到孕酮。

The changes of progesterone contents of ovary and mandibular organ in crayfish, Procambarus clarkii during the vitellogenesls were studled by using of radioimmunoassay. The progesterone contents increased at early second vitellogenesis stage and decreased significantly at the mid second vitellogenesis stage; the progesterone content of post spawning stage was undetective. These results indicated that the ovarian progesterone contents decreased during the mid second vitellogenesis, maturation and spawning. These findings suggest that progesterone content increase is related to the onset of the vitellogenesis. The progesterone content in mandiburlar organs at different developing stage was all undetective.