PI3K/AKT信号传导通路对鼻咽癌细胞增殖和凋亡的影响

EFFECTS OF PI3K-AKT PATHWAY ON PROLIFERATION AND APOPTOSIS OF NASOPHARYNGEAL CARCINOMA CELL LINE(CNE-2Z)

目的研究PI3K/AKT信号传导途径对人低分化鼻咽癌细胞系在CNE-2Z生长、增殖和凋亡的作用。方法应用wort mannin抑制PI3K的活性,绘制生长曲线、MTT观察wort mannin对CNE-2Z细胞生长、增殖的作用,以免疫细胞化学分别检测wort mannin处理培养后的CNE-2Z细胞中PI3K、AKT和bcl-2的表达,RT-PCR检测wort mannin处理后的细胞bcl-2 mRNA的表达情况,ELISA法检测wort mannin处理后的CNE-2Z细胞中的磷酸化的AKT的含量,流式细胞仪检测wort mannin处理培养后的CNE-2Z细胞的凋亡。结果 wort mannin对CNE-2Z细胞生长、增殖具有抑制作用,而且呈剂量依赖性。wort mannin作用后CNE-2Z细胞的PI3K和bcl-2的蛋白表达均减弱,而AKT的表达无明显变化。wort-mannin抑制CNE-2Z细胞bcl-2 mRNA的表达。wort mannin能显著抑制磷酸化的AKT的表达,并呈一定的剂量依赖性。Wort mannin还可以诱导CNE-2Z细胞的凋亡。结论 PI3K的抑制剂wort mannin可以有效抑制鼻咽癌CNE-2Z细胞的生长和增殖,诱导CNE-2Z细胞凋亡。

Objective To investigate the effects of the PI3K/AKT pathway on the growth and proliferation on CNE-2Z cells.Methods PI3K activity was inhibited by wortmannin（WT）.Cell growth curves and MTT assay were used to identify the effect of WT on the growth and proliferation of CNE-2Z cells.AKT and PI3K protein of CNE-2Z cells treated with WT were detected by IHC.P-AKT was evaluated by ELISA.Apoptotic features in CNE-2Z cells treated with the wortmannin were analyzed by FCM.Results The growth and proliferation of CNE-2Z cells were inhibited significantly by compared with the control group,which was in a dose-dependent manner.The expression of PI3K in the WT group was lower than that in the control group.However,the expression of AKT was not significantly decreased.The expression of P-AKT reduced significantly in a dose-dependent manner.Conclusion WT could inhibit the growth and proliferation and induce the apoptosis of CNE-2Z cells.