虎杖中白藜芦醇的酶法制备

Resveratrol Extraction from Polygonum cuspidatum with Enzymic Dissolution

以白藜芦醇得率为指标,通过对酶制剂的筛选和酶解条件的考察,分别选出酶法提取和酶法转化制备虎杖中白藜芦醇的最佳条件,并对两种酶法进行比较。结果显示:(1)酶法提取最佳酶解条件为:酶解温度45℃,酶解时间60 min,最适pH 5.0,底物浓度17%;酶法转化最佳酶解条件为:酶解温度40℃,酶解时间48 h,最适pH 5.0,底物浓度15%。(2)与空白样品(不加酶)相比,酶法提取白藜芦醇得率增加了3.12 mg/g;酶法转化白藜芦醇得率增加了12.72 mg/g。研究表明,两种方法与不加酶提取相比均提高了白藜芦醇的得率,但酶法转化效果更显著,可用于白藜芦醇的制备。

The best conditions of two enzymatic methods（Enzymatic extraction,Enzymatic conversion） to extract resveratrol from Polygonum cuspidatum were investigated in this paper.The results showed that：the optimum conditions for enzymatic extraction are hydrolysis temperature,45℃;hydrolysis time,60 min;pH 5.0 and substrate concentration,17%;The optimum conditions for enzymatic conversion are hydrolysis temperature,40℃;hydrolysis time,48 h;pH 5.0 and substrate concentration,15%.Compared with the blank sample（without enzyme）,the yield of resveratrol in enzymatic extraction increased 3.12 mg/g;The yield of resveratrol in enzymatic conversion increased 12.72 mg/g.Both enzymatic methods increased the yield of resveratrol.However,enzymatic conversion is more significant than enzymatic extraction.Thus enzymatic conversion method can be used to extract resveratrol.