小鼠下腔静脉移植模型的建立和改良

Construction and Improvement of Inferior Vena Cava Vein Graft Model in Mouse

目的建立异体下腔静脉移植于颈动脉的动物模型,以模拟冠状动脉旁路移植术后静脉桥再狭窄的病理过程。方法游离供体小鼠下腔静脉,切断受体小鼠右颈总动脉,实验组采用"袖套式"结扎吻合法、对照组采用缝合法将下腔静脉两端分别与颈总动脉断端行端端吻合。术后对比两组血管通畅情况,于术后1周、2周、4周、6周对实验组小鼠取材,行HE染色及免疫组织化学染色观察桥血管病理变化情况。结果实验组1例麻醉过量死亡,手术完成即刻各吻合口均通畅,无出血、狭窄及血管扭曲等情况。术后2例急性血栓形成,手术平均耗时46.0±10.6 m in;对照组手术完成即刻2例近端吻合口出血死亡,术后2例急性血栓形成,手术平均耗时68.0±12.4m in;后期静脉桥管壁逐渐增厚,管腔逐渐狭窄,HE染色及免疫组织化学染色显示中膜、内膜不同程度增生及炎症细胞浸润。结论小鼠异体下腔静脉移植模型能真实反映冠状动脉旁路移植术后静脉桥狭窄的情况,是进一步研究移植静脉内膜增生的理想模型。"袖套式"结扎吻合法建立小鼠下腔静脉移植模型,较以往常用的吻合法时间短、出血少、通畅率高。

Aim To construct vein grafts restenosis model in the C57BL/6 mouse to mimic graft restenosis after coronary artery bypass grafting. Methods Under anesthesia,the inferior Vena cava in mice was harvested and grafted into the carotid artery in ＂non-suturing sleeve pattern＂ with a cuff or in interrupted stich.Patency of grafts was evaluated respectively.Animals were sacrificed at the 1 week,2 weeks,4 weeks and 6 weeks postoperation,and the grafted tissue fragments were stained with HE and immunohistochemically. Results In the experimental group 1 mouse died due to overdosage of pentobarbital,all grafts were patent without distortion and leakage immediately after procedure and 2 grafts failed due to thrombosis,the average operation time was 46.0±10.6 min.In the control group 2 mice died from hemorrhage immediately after procedure,2 grafts failed due to thrombosis,the average operation time was 68.0±12.4 min.Vessel wall thickening was observed and progressed gradually at the time of harvest,which resulted in growing narrow of graft lumen.Histological analysis demonstrated varying degrees of neointimal hyperplasia and inflammatory cells. Conclusion The inferior Vena cava vein graft model established by using sleeving,which is more efficient and less leaky with higher graft patency when compared to interrupted stich,may be an ideal model for further study on the prevention of vein intimal hyperplasia after bypass grafting.