老年脑梗死患者血清对氧磷酶1活性与氧化应激的关系及其在动脉粥样硬化中的作用

Relationship Between Serum Paraoxonase-1 and Oxidative Stress in Elderly Patients with Cerebral Infarction and Their Roles in Atherosclerosis

目的探讨老年脑梗死患者血清对氧磷酶1活性与氧化应激的关系及其在动脉粥样硬化中的作用。方法通过DSA检查确诊颈动脉狭窄的老年脑梗死患者72例作为研究对象(其中轻度狭窄33例,中度狭窄24例,重度狭窄15例),以非脑血管病健康体检者38例作为对照组,用分光光度计法、黄嘌呤氧化酶法和硫代巴比妥酸反应物质法分别测定血清对氧磷酶1活性、超氧化物歧化酶和丙二醛含量,分析对氧磷酶1活性与超氧化物歧化酶、丙二醛之间的关系。结果脑梗死组患者血清对氧磷酶1活性(95.62±18.26 ku/L)和超氧化物歧化酶含量(69.59±10.56 ku/L)显著低于对照组(分别为168.36±27.82 ku/L和98.34±13.42 ku/L,P<0.01],而丙二醛含量(24.46±5.68 nmol/L)显著高于对照组(15.64±8.26 nmol/L,P<0.01)。轻度、中度和重度狭窄组患者对氧磷酶1活性(分别为112.48±19.32、98.64±10.84和81.95±13.42 ku/L)、超氧化物歧化酶(分别为80.62±13.26、70.26±14.09和58.82±10.06 ku/L)和丙二醛含量(分别为19.52±8.48、23.56±9.81和27.28±9.89 nmol/L)差异均有显著性(均P<0.01),并且对氧磷酶1活性和超氧化物歧化酶含量随颈动脉狭窄程度加重,呈逐渐下降的趋势,而丙二醛含量随颈动脉狭窄程度加重呈上升趋势。相关分析表明,对氧磷酶1活性与超氧化物歧化酶含量正相关(r=0.628,P<0.01),而与丙二醛含量负相关(r=-0.541,P<0.01)。结论老年脑梗死患者血清对氧磷酶1活性降低及氧化应激增强,对氧磷酶1活性和氧化应激及其复杂的相互作用参与动脉粥样硬化发生和发展。

Aim To investigate the relationship between serum paraoxonase-1（PON-1） and oxidative stress in elderly patients with cerebral infarction and their roles in atherosclerosis. Methods By DSA examination,72 elderly patients with cerebral infarction combined carotid artery atherosclerosis were involved in the study.The light stenosis was seen in 33 cases,the middle stenosis was seen in 24 cases,the heavy stenosis was seen in 15 cases.38 healthy people without cerebrovascular disease were adopted as control group.The activity of serum PON-1 was determined by spectrophotometer.The levels of serum superoxide dismutase（SOD） were detected by xanthine oxidase methods.The levels of serum malondial dehyde（MDA） were detected by thiobarbituric acid reactive substance.We have analyzed correlation between PON-1,SOD and MDA. Results The activity of serum PON-1 and the levels of serum SOD in the cerebral infarction group were significantly lower than those in the control group（95.62±18.26 ku/L vs 168.36±27.82 ku/L,69.59±10.56 ku/L vs 98.34±13.42 ku/L,P0.01）,while the levels of serum MDA in the cerebral infarction group were significantly higher than those in the control group（24.46±5.68 nmol/L vs 15.64±8.26 nmol/L,P0.01）.There were significant differences in the activity of serum PON-1（112.48±19.32 ku/L vs 98.64±10.84 ku/L vs 81.95±13.42 ku/L）,the levels of serum SOD（80.62±13.26 ku/L vs 70.26±14.09 ku/L vs 58.82±10.06 ku/L） and MDA（19.52±8.48 nmol/L vs 23.56±9.81 nmol/L vs 27.28±9.89 nmol/L） in the light,middle,heavy stenosis groups（P0.01）.The activity of serum PON-1 and the levels of serum SOD in cerebral infarction group decreased with the progression of carotid artery stenosis,while the levels of serum MDA increased with the progression of carotid artery stenosis.The correlation analysis results showed that there was positive correlation between the activity of PON-1and levels of SOD（r=0.628,P0.01）,while there was negative correlation between the activity of PON-1 and levels of MDA（r=-0.541,P0.01） in cerebral infarction groups. Conclusion The activity of PON-1 was significantly decreased,while oxidative stress was increased in elderly patients with cerebral infarction.The complicated interaction between oxidative stress and PON-1 may result in occurrence and development of atherosclerosis.