肝细胞肝癌中转录辅抑制因子CtBP1与其靶基因E-cadherin的表达

Expression of transcriptional corepressors CtBP1 and its target gene E-cadherin in hepatocellular carcinoma

目的探讨转录辅抑制因子CtBP1在肝细胞肝癌中的表达状况及对其靶基因E-cadherin表达的影响。方法采用免疫组化方法检测肝细胞肝癌组织芯片中CtBP1及E-cadherin的表达。结果 CtBP1在肝细胞肝癌组织中表达阳性率为79.69%(204/256),其表达与分化程度相关(P=0.049);E-cadherin在肝细胞肝癌组织表达率为20.31%(52/256),与肿瘤分级相关(P=0.027);CtBP1在肝细胞肝癌组织中表达与E-cadherin呈负相关(P=0.036)。结论在肝细胞肝癌组织中,CtBP1和E-cadherin可以作为肿瘤诊断的分子标记;同时转录辅抑制因子CtBP1对其靶基因E-cadherin的表达有抑制作用。

Purpose To investigate the expression of transcriptional corepressors CtBP1 in hepatocellular carcinoma and its impact on the expression of the target E-cadherin.Methods The expression of CtBP1 and E-cadherin protein in hepatocellular carcinoma tissues was assessed by immunohistochemical assay.Results The positive expression rates of CtBP1 and E-cadherin in hepatocellular carcinoma tissues were 79.69%（204/256） and 20.31%（52/256）,respectively.Both CtBP1 and E-cadherin were associated with the grade of tumor but not with other clinicopathological parameters.The expression of CtBP1 was negatively correlated with that of E-cadherin.Conclusions Transcriptional corepressor CtBP1 can suppress the expression of E-cadherin in hepatocellular carcinoma.Meanwhile,CtBP1 may be a potential diagnostic marker in grading of heptocellular carcinoma.