HIF-1α与CDX2在结直肠腺癌组织中的表达及意义

Expressions of HIF-1α and CDX2 and Its Clinical Pathological Significance in Colorectal Adenocarcinoma

目的探讨缺氧诱导因子-1α(HIF-1α)与基因系尾型同源盒基因2(CDX2)蛋白在结直肠腺癌组织中的表达及其与结直肠癌发生、发展的关系,并且研究HIF-1α与CDX2表达之间的相关性。方法收集62例结直肠腺癌及20例正常结直肠组织标本,采用免疫组化SABC法检测标本中HIF-1α、CDX2蛋白的表达,并采用Spearman等级相关分析分析HIF-1α与CDX2表达之间的相关性。结果在20例正常结直肠黏膜组织中HIF-1α及CDX2蛋白表达阳性率分别为5.0%(1/20)及95.0%(19/20),在62例结直肠腺癌组织中HIF-1α及CDX2蛋白表达阳性率分别为62.9%(39/62)及69.4%(43/62),不同组织中蛋白表达的差异均有统计学意义(P<0.05)。结直肠腺癌中,HIF-1α、CDX2蛋白的表达与肿瘤的分化程度、淋巴结转移及Dukes分期有关(P<0.05);Spearman等级相关分析表明,HIF-1α与CDX2的表达呈负相关(r=-0.293 2,P<0.05)。结论 HIF-1α表达上调、CDX2表达下调可能与结直肠腺癌的发生有关,且这两者间呈负相关。HIF-1α可能参与调控CDX2的表达,进而加速了结直肠癌的恶性进展。

Objective To investigate the expressions of hypoxia-inducible factor-1α（HIF-1α） and caudal homeobox gene 2（CDX2） in colorectal adenocarcinoma,and the relationships between them and the clinicopathologic factor of colorectal adenocarcinoma.Methods The expressions of HIF-1α and CDX2 were detected by immunohistochemistry in 62 specimens of colorectal adenocarcinoma and 20 specimens of normal colorectal mucosa tissue.The correlation between the expressions of HIF-1α and CDX2 was analyzed by Spearman rank correlation analysis.Results The positive rates of HIF-1α expression in normal colorectal mucosa tissue and colorectal adenocarcinoma were 5.0%（1/20） and 62.9%（39/62）,CDX2 were 95.0%（19/20） and 69.4%（43/62）,the differences of positive rate between different tissues were significant（P0.05）.In colorectal adenocarcinoma,the expression of HIF-1α or CDX2 was related to tumor differentiation,lymph node metastasis,and Dukes staging（P0.05）.There was a negative correlation between HIF-1α and CDX2 expressions in colorectal adenocarcinoma（r=-0.293 2,P0.05）.Conclusions The up-regulation of HIF-1α and down-regulation of CDX2 may be involved in the genesis of colorectal adenocarcinoma,and there is a negative correlation between the two kinds of protein.HIF-1α may participate in modulation of CDX2 expression and lead to accelerate the progression of colorectal carcinoma.