摘要
目的探讨缺氧诱导因子-1α(HIF-1α)与基因系尾型同源盒基因2(CDX2)蛋白在结直肠腺癌组织中的表达及其与结直肠癌发生、发展的关系,并且研究HIF-1α与CDX2表达之间的相关性。方法收集62例结直肠腺癌及20例正常结直肠组织标本,采用免疫组化SABC法检测标本中HIF-1α、CDX2蛋白的表达,并采用Spearman等级相关分析分析HIF-1α与CDX2表达之间的相关性。结果在20例正常结直肠黏膜组织中HIF-1α及CDX2蛋白表达阳性率分别为5.0%(1/20)及95.0%(19/20),在62例结直肠腺癌组织中HIF-1α及CDX2蛋白表达阳性率分别为62.9%(39/62)及69.4%(43/62),不同组织中蛋白表达的差异均有统计学意义(P<0.05)。结直肠腺癌中,HIF-1α、CDX2蛋白的表达与肿瘤的分化程度、淋巴结转移及Dukes分期有关(P<0.05);Spearman等级相关分析表明,HIF-1α与CDX2的表达呈负相关(r=-0.293 2,P<0.05)。结论 HIF-1α表达上调、CDX2表达下调可能与结直肠腺癌的发生有关,且这两者间呈负相关。HIF-1α可能参与调控CDX2的表达,进而加速了结直肠癌的恶性进展。
Objective To investigate the expressions of hypoxia-inducible factor-1α(HIF-1α) and caudal homeobox gene 2(CDX2) in colorectal adenocarcinoma,and the relationships between them and the clinicopathologic factor of colorectal adenocarcinoma.Methods The expressions of HIF-1α and CDX2 were detected by immunohistochemistry in 62 specimens of colorectal adenocarcinoma and 20 specimens of normal colorectal mucosa tissue.The correlation between the expressions of HIF-1α and CDX2 was analyzed by Spearman rank correlation analysis.Results The positive rates of HIF-1α expression in normal colorectal mucosa tissue and colorectal adenocarcinoma were 5.0%(1/20) and 62.9%(39/62),CDX2 were 95.0%(19/20) and 69.4%(43/62),the differences of positive rate between different tissues were significant(P0.05).In colorectal adenocarcinoma,the expression of HIF-1α or CDX2 was related to tumor differentiation,lymph node metastasis,and Dukes staging(P0.05).There was a negative correlation between HIF-1α and CDX2 expressions in colorectal adenocarcinoma(r=-0.293 2,P0.05).Conclusions The up-regulation of HIF-1α and down-regulation of CDX2 may be involved in the genesis of colorectal adenocarcinoma,and there is a negative correlation between the two kinds of protein.HIF-1α may participate in modulation of CDX2 expression and lead to accelerate the progression of colorectal carcinoma.
出处
《中国普外基础与临床杂志》
CAS
2010年第12期1265-1269,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金资助项目(项目编号:30672070
30400430)
陕西省科技计划资助项目(项目编号:2009K12-01
2009K01-70)
西安交通大学医学院第一附属医院科研基金资助项目(项目编号:2009YK03)
光华医学创新研究基金资助项目(项目编号:0203126)~~