血管内皮生长因子基因多态性与脑梗死的关系

Correlation of VEGF gene polymorphism with cerebral infarction

目的:研究血管内皮生长因子(VEGF)基因rs1570360、rs3025039和rs699947与脑梗死是否存在相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对147例急性脑梗死患者(脑梗死组)和131例非急性脑梗死对照者(对照组)VEGF基因rs1570360、rs3025039和rs699947的多态性进行分析。结果:脑梗死组的三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、血糖、血压(收缩压、舒张压)明显高于对照组,而高密度脂蛋白胆固醇(HDL-C)则明显降低(P均<0.05)。脑梗死组患者VEGF rs1570360、rs3025039和rs699947基因型频率和等位基因频率与对照组比较,差异无统计学意义(P>0.05)。脑梗死危险因素的多元逐步Logistic回归分析显示,TG、血糖和收缩压是脑梗死的独立危险因素。然而,脑梗死组患者VEGF rs1570360、rs3025039和rs699947的单倍型ACC(5.85%)明显低于对照组(14.22%)。结论:rs1570360、rs3025039和rs699947的单倍型ACC与脑梗死的发病可能有一定的关系。

Objective To study whether vascular endothelial growth factor（VEGF）gene polymorphism has correlation with the risk of cerebral infarction.Methods PCR-RFLP was used to analyze the polymorphism of VEGF gene rs1570360,rs3025039 and rs699947 in 147 cases of acute cerebral infarction and 131 cases of non-cerebral infarction control.Results TG,LDL-C,blood glucose,blood pressure（systolic and diastolic pressure） were obviously higher in cerebral infarction group than those in control group,and HDL-C was obviously lower（P0.05）.There was no significant difference in allele and genotype distributions of rs1570360,rs3025039 and rs699947 between cerebral infarction group and control group（P0.05）.Multi-step logistic regression analysis of cerebral infarction risk factor showed that TG,blood glucose and systolic pressure were the independent risk factor,however,haplotype ACC of rs1570360,rs3025039 and rs699947（5.85%） in cerebral infarction group was obviously lower than that in control group（14.22%）.Conclusions Haplotype ACC of rs1570360,rs3025039 and rs699947 might have some correlation with the risk of cerebral infarction.