铁调素对成骨细胞骨相关基因表达的影响

The Effects of hepcidine on osteoblast-related gene expression

目的研究铁调素对人成骨细胞(hFOB1.19)Ⅰ型胶原(COL1)、骨保护素(OPG)、骨钙素(BGP)基因表达的影响。方法成骨细胞在34℃下进行体外培养,以不同浓度铁调素(50 nmol/L、100 nmol/L、200 nmol/L、400 nmol/L)干预72 h后,收集细胞,分别抽取总RNA,采用半定量RT-PCR法检测成骨细胞中COL1、OPG、BGP mRNA表达的变化。结果 RT-PCR检测显示不同浓度铁调素干预后,各组COL1、OPG、BGP mRNA均有表达;不同浓度组的COL1、OPG、BGP mRNA表达光密度比值不同,组间密度比值比较存在统计学意义(P<0.05),但铁调素在400 nmol/L时抑制OPG的表达(P<0.05)。结论 铁调素可上调成骨细胞(hFOB1.19)COL1、OPG、BGP mRNA表达,铁调素浓度增加转录水平逐渐增加,显示有浓度依赖性。

Objective To investigate the effects of hepcidin on the gene expressions of osteoprotegerin （OPG） , Collagen-I （COL1） , and osteocalcin （BGP） in human hFOB1.19 osteoblasts in vitro. Methods The osteoblasts were cultured at 34℃ in vitro. The cultures were additioned with different concentrations of hepcidin （50 nmol/L, 100 nmol/L, 200 nmol/L, and 400 nmol/L, respectively） for 72 h. The cells were collected and the total RNA was isolated. Expressions of COL1, OPG, and BGP mRNA were detected with semiquantitative RT-PCR. Results The result of RT-PCR showed that the mRNAs of COL1, OPG, and BGP were all expressed after the intervention of hepcidine with different concentrations. The density ratios of COL1, OPG, and BGP mRNA differed among the different hepcidin concentration groups, which showed statistical differences （P 〈 0.05）. Hepeidin inhibited the mRNA expression of BGP at the concentration of 400 nmol/L （P 〈 0.05）. Conclusion Hepcidin upregulated the expression of COL1,0PG and BGP mRNA in hFOB1.19 osteoblasts. Hepcidine dose-dependently stimulated the level of transcription.