硝化污泥保藏特性的研究

Influence of storage on the characteristics of nitrifying sludge

研究了不同保藏方法对硝化污泥硝化活性和相关性状的影响.结果表明,不同饥饿保藏条件下,各种方法下的硝化活性均呈指数衰减,自然环境、4℃、-20℃、-20℃加15%体积甘油方法下的活性衰减速率分别为0.012d-1、0.021d-1、0.019d-1、0.013d-1,半衰期分别为1.71、1.09、1.22、1.62个月.保藏5个月后,4种方法下的硝化污泥活性保留率分别为16.0%、13.6%、20.8%、20.4%,菌体保留率分别为41.5%、47.6%、52.2%和53.6%.保藏过程对硝化细菌的ATP含量、血红素c含量、SV值均产生影响,随着保藏时间的延长,低温下污泥中的丝状菌比例增加,絮体结构更加紧密,硝化污泥变黑速度减慢,而自然环境中丝状菌消失,絮体结构解体,硝化污泥迅速变黑.

The preservation characteristics of nitrifying sludge under different storage methods were investigated. Under starvation conditions in the natural environment,compared to 4 ℃,-20 ℃ and-20 ℃ with 15% glycerol,the decay rate of nitrifying activity was 0.012 d-1,0.021 d-1,0.019 d-1 and 0.013 d-1,and the half-life was 1.71,1.09,1.22 and 1.62 months,respectively. After 5 months＇ storage,the remaining relative activity and relative biomass were 16.0%,13.6%,20.8%,20.4% and 41.5%,47.6%,52.2%,53.6%,respectively. During storage,the hemecand ATP contents declined,and the SV also decreased with the decrease of activity. When storage time was extended,at low temperature,filamentous bacteria dominated in the sludge,the biomass structure became integrated,and the nitrifying sludge changed to black gradually. Under natural conditions,filamentous bacteria decreased,the sludge structure became loose,and the nitrifying sludge turned black rapidly.