多金属污染土壤中微生物群落功能对麻疯树-化学联合修复的响应

Response of soil microbial community function to chemical-aided remediation of multi-metal contaminated soils usingJatropha curcas

应用BIOLOG实验技术,结合土壤和植物的相关指标,对广东省大宝山矿山周边多金属污染土壤修复过程中微生物群落代谢方式的变化进行分析,结果表明,施用改良剂可以显著提高土壤微生物群落的活性:粉煤灰>石灰>白云石>对照,其中粉煤灰对提高多金属污染土壤微生物的代谢多样性有显著效果;种植麻疯树后土壤微生物活性也发生变化,结果为:白云石>石灰>粉煤灰>对照,白云石和麻疯树联合使用修复效果更显著;白云石可以刺激根系分泌L-丝氨酸、4-羟基苯甲酸、L-精氨酸、D-纤维二糖、β-甲基—D-葡萄糖苷、D-甘露醇、D-半乳糖醛酸等碳源;不同处理的土壤微生物对糖类、其它类、氨基酸类和胺类等碳源利用能力强的,能促进麻疯树生长并抑制对重金属的吸收.

Chemical amendments were made to multi-metal contaminated acid soils of Dabaosan mine sites with Jatropha curcasand the response of the microbial communities in the soil was investigated. The application of chemical amendments significantly improved the activity of soil microbial communities without J. curcas（flyash limestone dolomite control）. However,the activity of microbial communities altered in the presence of J. curcasin the order dolomite limestone flyash control. The combination of dolomite with Jatrophasignificantly improves soil remediation in terms of stimulating root exudates and other metabolites such as L-serine,4-hydroxy benzoic acid,L-arginine,D-cellobiose,β-methyl-D-glucoside,D-mannitol and D-galacturonic acid. The soil microbial communities have a strong ability to utilize saccharides,amino acids and amines to promote growth and inhibit the uptake of metals in J. curcas. Based on these results,we conclude that the combination of chemical amendments with Jatrophaenhances the remediation potential in terms of altered microbial community profiles in multi-metal contaminated acid soils.