基因组重排与传统育种相结合选育大观霉素高产菌株

Genome shuffling combined traditional mutagenesis for improving Streptomyces spectabilis

以壮观链霉菌(Streptomyces spectabilis)为研究对象,采用基因组重排技术与传统诱变育种相结合的方法选育大观霉素的高产菌株。通过原生质体紫外诱变获得壮观链霉菌突变体群体,高产突变菌株间进行两轮的基因组重排,筛选的高产菌株用NTG诱变得新霉素和链霉素的抗性突变菌株,抗性突变菌株间进行两轮基因组重排,从双抗突变菌株中筛选到一株大观霉素产量为4405U/mL的突变菌株LXR1H-8,其产素水平较原始出发菌株提高了486.3%。

The genome shuffling combined the traditional mutagenesis was applied to improve Streptomyces spectabilis for producing spectinomyein. Firstly the UV-irradiation was adopted to mutate the protoplasts of Streptomyces spectabilis, and then the mutant strains with high specinomycin production were carried to protoplast fusion by twice. The fusant strain BR2-1 was screened out and sent to NTG-induced mutation. The neomycin-resistant strains X40-2, X40-5 and streptomycin-resistant stains L30-1, L50-1 were selected to send to the second genome shuffling from the NTG-treated filial strains. Lastly a fusant strain LXR1H-8 producing spectinomycin as high as 4 405 U/mL was obtained, resistant to both neomycin and streptomycin. The yield of spectinomycin was increased by 486.3 % comparing with that of the original strain XZ-4.