角蛋白降解菌的筛选及其UV诱变选育

Screening of keratin-degrading strain and its UV mutational breeding

从长年堆积废羽毛的土壤,屠鸡场下水道、池塘底污泥中分离出一株角蛋白降解菌C-1,摇瓶发酵测得其角蛋白酶活达35.6U,经形态观察和生理生化鉴定该菌株属于芽孢杆菌属。对菌株进行UV诱变,在照射时间为100s下得到一株产角蛋白酶活较高突变株C-1-4,其酶活比原始出发菌株提高了122%,且遗传性状稳定。

One keratin-degrading bacterium named C-1 was isolated from the abandoned feather soil piled for long years and the mud from both pool and poultry keeping yard. Its keratinase activity reached 35.6 U. Through the morphological observation, physiological and biochemical experiments, the strain was preliminarily identified as Bacillus sp. In addition, the strain C-1 was dealed with UV mutation and the strain C-1-4 with higher enzyme activity was obtained by 100 s UV irradiation. The enzyme activity of C-1-4 was 122% more than that of the original strain.