响应面法优化产丙酸杆菌素菌株的培养条件

Optimization of propionin production by Response Surface Method

响应面法已成功运用于发酵过程的优化,可以对发酵培养基及培养条件等诸多因素进行考察和评价,从而有效地确定重要因子的最优水平,取得更好的发酵效果,因此本文主要采用响应面法对丙酸杆菌素产生菌株的发酵培养基进行优化,以提高丙酸杆菌素抑菌能力。首先采用Plackett-Burman(PB)试验设计法筛选出3个主要因素为葡萄糖、Tween80和pH值,然后通过最陡爬坡法和响应面分析方法确定主要因素的最佳质量浓度分别为:葡萄糖2.32 g/L,Tween80 0.1 g/L,pH值为7.0。在优化条件下丙酸杆菌素抑菌能力增强,形成的抑菌圈直径是24.76 mm,是优化前的1.25倍。

Fermentation process were optimized by using Response Surface Analysis method, the fermentation medium and the cultural conditions can be Examined and evaluated, optimal levels of the variables also can be defined ,then the Fermentated activity can beimproved, the fermentation medium for propionin production was optimized by this methed in order to improve the inhibitory activity of propionin. Firstly, three statistically significant parameters including glucose, tween80, pH were identified by Plackett-Burman design. The optimal levels of three variables were defined by Steepest ascent method and Response surface method（RSM）： glucose 2.32 g/L, tween80 0.1 g/L, pH 7.0. The inhibitory activiW under the optimal fermentation medium can be improved. The diameter of inhibition zone was 24.76 mm, which was as 1.25 times as that under the original fermentation medium.