中药半枝莲黄酮类成分的分离与结构鉴定

Isolation and identification of flavonoids of whole plant of Scutellaria barbata D.Don

目的对中药半枝莲(Scutellaria barbata D.Don)全草的化学成分进行研究。方法应用大孔树脂、正相硅胶、反相ODS、Sephadex LH-20以及制备型高效液相等色谱法对中药半枝莲的化学成分进行了分离;并通过理化性质和光谱数据对化合物的结构进行了鉴定。结果分离鉴定了7个黄酮类化合物,分别为芹菜素(apigenin1,)、木犀草素(luteolin,2)、5,7,4'-三羟基-8-甲氧基黄酮(5,7,4'-trihydroxy-8-methoxyflvone,3)、芹菜素-7-O-β-D-葡萄糖醛酸苷(apigenin-7-O-β-D-glucuronide,4)、芹菜素-7-O-β-D-葡萄糖醛酸甲酯(apigenin-7-O-β-D-glucuronide methyl ester,5)、芹菜素-7-O-β-D-葡萄糖苷(apigenin-5-O-β-D-glucopyranoside,6)、山奈酚-3-O-β-D-芦丁糖苷(kaempferol-3-O-β-D-rutino-side,7)。结论化合物4-7为半枝莲植物中首次分离得到。

Objective To study the chemical constituents of the whole plant of Scutellaria barbata D. Don. Methods The compounds were isolated by chromatographic methods including resin, silica gel, ODS, Sephadex LH-20 and preparation HPLC et al. , and identified by the chemical and physical methods, especially spectral analysis. Results Seven flavonoids were isolated. The structures of them were identified as apigenin （ 1 ）, luteolin （2）, 5,7,4＇-trihydroxy-8-methoxyflvone （3）, apigenin-7-O-β-D-glucuronide （4）, apigenin-7-O- β-D-glucuronide methyl ester （ 5 ）, apigenin-5 -O-β-D-glucopyranoside （ 6 ） and kaempferol-3-O-β-D-rutinoside （7）, respectively. Conclusions Compounds 4-7 are obtained from S. barbata for the first time.