火绒草黄酮类成分的分离与结构鉴定

Isolation and identification of Flavonoids of whole plant of Leontopodium leontopodioides(Willd.) Beauv.

目的对火绒草的化学成分进行研究,为进一步开发利用该植物资源提供依据。方法采用反复正相硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱法等手段进行分离纯化,并通过理化性质与光谱分析鉴定化合物的结构。结果从火绒草体积分数为70%的乙醇溶液提取物中分离鉴定了9个黄酮类化合物,分别为槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin-3'-O-β-D-gluco-pyranoside,1)、槲皮素-3'-O-β-D-葡萄糖苷(quercetin-3'-O-β-D-glucopyranoside,2)、5,7,3',4'-tetra-hydroxy-3-methoxyflavone(3)、5,7,3',4'-tetrahydroxy-3-methoxyflavonol-3'-O-β-D-glucopyranoside(4)、山柰酚(kaempferol5,)、山柰酚-3-O-β-D-吡喃葡萄糖苷(kaempferol-3-O-β-D-glucopyranoside6,)、3-甲基-山柰酚(3-methyl-kaempferol,7)、3-甲醚-山柰黄素-7-O-β-D-吡喃葡萄糖苷(3-methylether-kaempferol-7-O-β-D-glucoside,8)、木犀草素-3'-O-β-D-葡萄糖苷(luteolin-3'-O-β-D-glucoside9,)。结论化合物2-57、8、为首次从火绒草属植物中分离得到。

Objective To study the chemical constituents from the whole plant of Leontopodium leontopodioides （WiUd.） Beauv.. Methods The compounds were isolated by chromatographic methods such as silica gel, ODS, Sephadex LH-20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis. Results Nine flavonoids were isolated. The structures of them were identified as quercetin- 3-O-β-D-glucopyranoside （ 1 ）, quercetin-3＇-O-β-D-glucopyranoside （ 2 ）, 5,7,3＇, 4＇-tetrahydroxy-3-methoxyflavone （ 3 ）, 5,7,3＇, 4＇-tetrahydroxy-3-methoxyflavonol-3 ＇-O-β-D-glucopyranoside （ 4 ）, kaempferol （ 5 ）, kaempferol-3-O-β-D-glucopyranoside （ 6 ）, 3-methylkaempferol （ 7 ）, 3-methylether-kaempferol-7-O-β- D-glucoside （8）, luteolin-3 ＇-O-β-D-glucoside （ 9 ）, respectively. Conclusions Compounds 2- 5,7 and 8 are obtained from Leontopodium genus for the first time.