HPLC法测定人血清中CYP3A4探针药物氨苯砜的浓度

Determination of Cytochrome P_(450)3A4 Probe Drug Dapsone in Human Serum by HPLC

目的:建立测定人血清中细胞色素P4503A4(CYP3A4)探针药物氨苯砜浓度的方法。方法:采用高效液相色谱法,色谱柱果为迪:氨马苯D砜ia血mo药ns浓ilC度18在,流1动～相6m为g.乙L腈-1-范水(围2内5:线75性),关流系速良为好1(.0rm=L0..9m9i9n-1)1,;紫方外法检回测收波率长为为942.5985%nm～,柱10温2.7为7%30,℃日,内进、样日量间为RS30Dμ分L别。≤结7.05%、5.54%。结论:本方法快速、准确、灵敏度高、专属性强,适用于人血清中氨苯砜浓度的测定。

OBJECTIVE： To establish the method for determination of cytochrome P4503A4 （CYP3A4）probe drug dapsone in human serum by HPLC. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-water （25 ： 75） at flow rate of 1.0 mL. min^-1. UV detection wavelength was set at 258 nm and the column temperature was 30℃. The irdection volume was 30μL. RESULTS： The linear range of dapsone were 1-6 mg.L^-1（r= 0,999 1） with recovery rate of 94.95%-102,77%, The RSD of intra-day and inter-day were less than 7.05% and 5,54%, CONCLUSION： The method is rapid, accurate, sensitive and specific for concentration determination of dapsone in human serum.