重组人甲状旁腺素对慢性苯中毒小鼠造血损伤的改善作用

Improving Effect of rhPTH on Hematopoietic Depression in Mice Caused by Chronic Benzene Poisoning

[目的]研究重组人甲状旁腺素(rhPTH)对慢性苯中毒小鼠造血损伤的改善作用。[方法]皮下注射苯诱导小鼠造血系统损伤模型。将造模成功的30只慢性苯中毒小鼠随机分为3组:生理盐水治疗组、40μg/kg rhPTH治疗组、80μg/kg rhPTH治疗组。每周5次腹腔注射生理盐水或不同剂量rhPTH,治疗3周,间隔1周后取样,测定3组小鼠骨髓中的造血干细胞(外源性脾结节法)、造血祖细胞(集落培养)以及外周血细胞计数和脾脏系数。[结果]经rhPTH治疗的小鼠一般状况明显好于生理盐水治疗组;40μg/kg rhPTH治疗组与80μg/kg rhPTH治疗组的造血干细胞数明显高于生理盐水治疗组(P<0.05),但两个剂量rhPTH治疗组间造血干细胞数差异无统计学意义;3组小鼠的造血祖细胞及外周血细胞计数均差异无统计学意义;两个rhPTH治疗组的脾脏系数均低于生理盐水治疗组(P<0.05)。[结论]rhPTH可明显增加慢性苯中毒小鼠骨髓中造血干细胞数量。

L Objective j To explore the improving effect of recombinant human parathyroid hormone （ rhPTH ） on bone marrow hematopoietic depression in mice caused by chronic benzene poisoning. [ Methods ] The model mice of hematopoietic depression was established in a number of KM mice by subcutaneous administration of benzene. Established model mice were treated 5 times a week for 3 weeks intraperitoneally with normal saline （ NS group ）, 40 μg/kg rhPTH（ 40PTH group ）, and 80 μg/kg rhPTH（ 80PTH group ） separately. One week later, all mice in the three groups were sacrificed. The hematopoietic stem cell, hematopoietic progenitor cell, peripheral blood cell count, and spleen index were determined by routine methods. [ Results ] Mice in PTH-treated groups showed better general condition than mice in NS group. The hematopoietic stem cells in 40PTH group and 80PTH group were significantly increased than those in NS group （ P 〈 0.05 ）. However, the hematopoietic progenitor cells and peripheral blood counts had no significant differences among the three groups. The spleen index in 40PTH group and 80PTH group was notably lower than NS group（ P 〈 0.05 ）. [ Conclusion ] rhPTH markedly increased the number of hematopoietic stem cells in mice with chronic benzene poisoning.