不同提取方法测定野马追药材中绿原酸含量比较研究

Comparative Study of Different Extraction Methods to Determine Chlorogenic Acid Content in YMZ

目的:研究野马追药材中绿原酸含量测定的前提取方法及绿原酸含量的质控标准。方法:采用高效液相色谱法,比较了不同的提取方法、提取溶媒、提取时间和溶媒用量测定野马追药材中绿原酸含量。结果:采用超声提取方法提取野马追药材中绿原酸,且绿原酸高效液相法测定进样量在0.0908～1.362μg(r=0.9999)间呈良好的线性关系,平均回收率为100.12%,RSD为1.31%。结论:绿原酸超声提取方法和高效液相测定含量方法简便,可用于该药材的质量控制。

Objective：To study the YMZ in the content determination of chlorogenic acid in the extraction methods and quality control of chlorogenic acid standards.Methods：High performance liquid chromatography to compare the different extraction methods,extraction solvent,extraction time and solvent consumption wild chase determination of chlorogenic acid content of herbs.Results：The use of ultrasonic extraction method to recover the herbs in chlorogenic acid,and chlorogenic acid by HPLC with sampling in the 0.09081.362μg（r = 0.9999）showed a good linear relationship between the average recovery was 100.12%,RSD 1.31%.Conclusion：The ultrasonic extraction of chlorogenic acid content was determined by high performance liquid method is simple and can be used for quality control of the medicine.