摘要
为研究体外培养的大鼠松果体细胞分泌的松果体素是否具有内在节律,收集体外培养的大鼠松果体细胞在白天6∶00,8∶00,10∶00,12∶00,15∶00,和夜晚18∶00,20∶00,22∶00,1∶00,4∶00各个时间点的培养上清,并用放射免疫方法(RIA)检测各个时间点松果体细胞培养上清中的松果体素水平.CCF(complex cosine function)统计分析结果表明,在持续黑暗的环境下,体外培养的大鼠松果体细胞分泌的松果体素在培养3,6,9天后仍然表现出内在节律.然而,这种内在节律在培养12天后消失.上述结果提示体外培养的大鼠松果体细胞可以作为一个有用的体外模型来分析节律系统的生理基础.
To investigate whether rat pineal cell is capable of generating a circadian oscillation in melatonin release,the cultured pineal cell in culture medium was collected at 6∶00,8∶00,10∶00,12∶00 and 15∶00 during the day,and at 18∶00,20∶00,22∶00,1∶00 and 4∶00 at night respectively.The melatonin level in culture medium was measured by a direct radioimmunoassay.The complex cosine functions（CCF） statistical analysis indicates that the rat pineal cell melatonin synthesis shows an intrinsic rhythm at 3,6,9 day culture under constant dark conditions.However,the intrinsic rhythm disappears at 12 day culture.This result suggests that the rat individual pineal cells can function as a circadian oscillator in vitro.
基金
Supported by Natural Science Foundation of Anhui Province(090413122)
Doctoral Fund of Ministry of Education of China(20093402120028)
