小鼠胃和阑尾组织中h-CD降解规律与死亡时间的相关性研究

Associativity study between h-CD degradation regularity and the estimation of postmortem interval in stomach and appendix of mice

目的:观察小鼠死亡后不同时间胃和阑尾组织中h-CD含量的变化,探讨不同温度条件下h-CD的降解规律与死亡时间的关系及其组织差异性。方法:选取72只健康成年昆明小鼠,采用机械性窒息致死后,随机分为两组,分别置于4℃冰箱和25℃人工气候箱中,并于死后0、24、48、72、96、120 h提取胃及阑尾组织,采用免疫印记及计算机图像分析技术检测上述组织中h-CD的含量,应用SPSS 16.0软件对数据进行统计学分析。结果:①h-CD的含量随死亡时间的延长而降低,与死亡时间具有相关性;②不同温度条件下,胃和阑尾组织中h-CD的降解速度均存在明显差异(P<0.05),h-CD在25℃组降解速度快于4℃组(P<0.05);③h-CD的降解速度在胃和阑尾组织中存在组织差异性,在阑尾组织中的降解速度较快(P<0.05)。结论:h-CD的含量随死亡时间延长而降低,其降解速度受环境温度影响,温度升高,降解速度加快,且胃和阑尾组织中h-CD的降解速度具有组织差异性,其在阑尾组织中的降解速度快于胃组织中的降解速度。

Objective：To observe the postmortem degradation of high molecular-weight caldesmon（h-CD） in stomach and appendix smooth muscle cells of mice,study the effects of postmortem intervals,and ambient temperature on the h-CD degradation of mice,and the variability of the h-CD degradation in stomach smooth muscle cells and appendix smooth muscle cells in different ambient temperature.Methods：72 healthy adult Kunming mice were killed by asphyxia,and put them in 4℃ refrigerator and 25℃ artificial climate incubator respectively,then extractd the stomach and appendix tissues at 0,24,48,72,96,120 h after dead.The h-CD contents in the above tissues were quantitated by western-blot assay and collected by Image Pro Plus 5.0 image analysis system,then the datas were statistically analyzed with the SPSS 16.0 software.Results：①h-CD content in stomach and appendix tissues decreased with the postmortem intervals,there was correlation between the postmortem degradation of h-CD and the postmortem intervals;②The differences of the postmortem degradation of h-CD in different ambient temperature were significant（P0.05）,the degradation in 25℃ faster than that in 4℃;③The differences of the postmortem degradation of h-CD between stomach and appendix tissues were significant（P0.05）,the degradation in the appendix more faster.Conclusion：h-CD content in stomach and appendix tissues decreased gradually with the postmortem intervals,the postmortem degradation of h-CD is influenced by ambient temperature,and the degradation rate tends to be raised with higher temperature,and the differences of the postmortem degradation of h-CD between stomach and appendix tissues are significant,the degradation in the appendix more faster.