固体与液体培养法在鉴别支原体感染的差异性研究

Comparison in solid culture method and broth culture method on ureaplasma urealyticum and mycoplasma hominis

目的:通过固体和液体培养方法,比较解脲支原体和人型支原体在感染比例、特异性和敏感性等方面的差异性。方法:首先采用液体Uu和Mh培养试剂盒测试2010年1月～10月疑似生殖道支原体感染患者5 200例;然后对液体培养阳性和污染标本进行固体选择分离培养基培养并评估其特异性和敏感性。结果:5200例生殖道支原体感染患者,液体培养法实验结果:阳性1690例,占32.50%,阴性3 510,占67.50%,污染250例,占4.81%。其中,单纯Uu感染18.50%,单纯Mh感染2.23%(P<0.05)。1690例液体培养法阳性标本固体培养阳性1280例,差异率为24.26%(P<0.05),其中,单纯Mh差异性最大,占43.97%。250例液体污染标本,固体培养结果有典型支原体生长58例,差异性占23.20%。典型的支原体1:10倍比稀释培养,结果显示,固体培养基最高稀释阳性稀释倍数为(1×10-4),液体培养基阳性最高稀释倍数为(1×10-6),P<0.05。结论:泌尿生殖道支原体感染比例和种类差异较大,液体培养法可用于初筛,固体培养法可用于鉴定和确证试验。

Objective：To Compare with the Infec ratio and specificity and sensitivity by solid culture method and broth culture method with ureaplasma urealyticum and mycoplasma hominis.Methods：First,the gential tract mycoplasma infection 5 200 specimens to doubt were tested by broth method on ureaplasma urealyticum and mycoplasma hominis from January to October in 2010.And then,the resuits of positive and pollution specimens from broth method were analyzed by broth method,and evaluated the specificity and sensitivity respectively.Results：A total of 5200 patients were measured,and the positive rate of Mycoplasma was 1 690（32.50%）,negative was 3 510（67.50%）,pollution was 250（4.81%）,among them,the positive rate of Uu was 18.50%,Mh was 2.23%（P0.05）.The difference rate was 24.26% between solid method and broth method,among them,the rate of Mh was 43.97%,pollution was 23.20%.The sensitivity was（1×10-4） in solid method and（1×10-6） in broth method（P0.05）.Conclusion：There is a high positive rate and different species in the gential tract mycoplasma infection in clinic.It is a good choice to solid method with preliminary screening and broth method with identification and confirmatory test.