Ki-67在寻常型银屑病中的表达及其意义

Expression and significance of Ki-67 in psoriatic lesions

目的探讨Ki-67在寻常型银屑病发病中的作用。方法采用免疫组织化学LDP法检测了48例寻常型银屑病皮损区及20例正常人表皮中Ki-67的表达及分布特点,采用逆转录聚合酶链反应技术(RT-PCR方法)检测Ki-67的mRNA表达水平。结果寻常型银屑病皮损区Ki-67表达均较正常表皮中的表达明显增强,分布于除角质层以外的表皮各层,而正常表皮表达阴性或仅在基底层有弱阳性表达。RT-PCR检测结果表明寻常型银屑病组Ki-67的表达水平亦显著高于正常表皮。结论 Ki-67在寻常型银屑病皮损中过度表达,提示其与银屑病的表皮细胞过度增殖、分化异常有关。

Objective To investigate the effect of Ki-67 on the pathogenesis of psoriasis vulgaris lesions and to evaluate its possible significance.Methods The expression and distribution of Ki-67 were detected by immunohistochemistry in the lesions of 48 cases with psoriasis vulgaris and in the epidermis of 20 normal controls.The mRNA expression of Ki-67 was detected by RT-PCR methods.Results The expressions of Ki-67 in psoriatic epidermis were significantly higher than those in normal epidermis;they were located in all epidermal layers except horny layer.There were no or only weak positive expressions in the basal layer of normal controls.Ki-67 mRNA were markedly increased in psoriasis vulgaris.Conclusion The over expressions of Ki-67 in psoriatic epidermis suggest that it might be related to the hyperproliferation and abnormal differentiation of psoriatic keratinocytes.Ki-67 may be a novel target in preventing the process of psoriasis vulgaris.