LY294002对人胃癌HGC-27细胞增殖的影响

Effect of LY294002 on the proliferation of gastric carcinoma cell HGC-27

目的探讨PI3K特异性抑制剂LY294002对胃癌HGC-27细胞增殖的影响。方法用不同浓度(0、10、20、40μmol.L-1)的LY294002对细胞进行干预至不同的时间(20、40、60 h),CCK-8法检测细胞增殖,流式细胞仪(PI染色)检测细胞周期。结果 LY294002作用于HGC-27细胞后,不同浓度的LY294002均对人胃癌HGC-27细胞具有明显的抑制生长作用,并随着剂量的增加而逐渐增强(P=0.000);当LY294002浓度为40μmol.L-1时,LY294002对HGC-27细胞的抑制作用随着培养时间的延长而逐渐增强(P=0.000)。细胞周期分析显示LY294002作用60 h后,呈浓度依赖性改变细胞周期的分布,G0/G1期细胞比例逐渐上升,S期细胞比例逐渐下降,G2/M期细胞比例逐渐增加,细胞周期发生G0/G1期、G2/M期阻滞。结论 LY294002作用于HGC-27细胞后,能够抑制细胞增殖和细胞周期的进展。

Aim To investigate the effects of the specific PI3K inhibitor LY294002 on the proliferation in gastric carcinoma HGC-27.Methods The HGC-27 cells were incubated with different concentrations（0,10,20 and 40 μmol·L-1 separately） of LY294002 for different times（20,40 and 60 h separately）.CCK-8 was taken to test the cell proliferation.Propidium Iodide dying was taken to test the dislocation of cell cycle.Results After the HGC-27 cells were treated with LY294002,the cell proliferation was inhibited,and the effect of proliferation inhibition was increased with the concentrions（P=0.000）.The effect of proliferation inhibition was increased with interfering times when the concentrions of LY294002 was 40 μmol·L-1（P=0.000）.The cell cycle analysis showed the dose-dependent manner,the rate of G0/G1 and G2/M stage cells was increased,and the rate of S stage cells was decreased with concentrions when the interfering time was 60 h（P=0.000）.Conclusion LY294002 can inhibit the proliferation and the cell cycle progression of HGC-27 cells.