摘要
选用苯胺及其几个衍生物作聚合单体，用电化学聚合方法在玻碳电极上固定辣根过氧化物酶制备了测定Ｈ２ Ｏ２ 的传感器，经比较筛选出最灵敏的聚合单体Ｎ，Ｎ－ 二甲基苯胺进行了详细的研究。传感器的最佳操作电位为－ ０．０５Ｖ，在此电位下，常见的电活性物质在传感器上响应很小，因此干扰较小。传感器具有较高的灵敏度，对Ｈ２ Ｏ２ 的线性响应范围为１×１０－ ８ ～１×１０－ ５ ｍｏｌ／Ｌ，检测限达５×１０－ ９ ｍｏｌ／Ｌ。
Aniline and some of its derivatives are chosen as monomers for electrochemical immobilization of horseradish peroxidase on glassy carbon electrode to prepare H2O2 biosensors. The best monomer, N,N-diamethylaniline, is selected based on the experiments and the sensor prepared with this monomer is fully investigated. The optimum operating potential of the sensor is -0.05V. With this potential, the response of the electroactive species commonly encountered in physiological samples is very small, so the interference from these substances is minimum. The sensor gives a linear response to H2O2 in a range of 1×10-8～1×10-5 mol/L and a detection limit of 5×10-9 mol/L.
出处
《苏州城建环保学院学报》
1999年第3期17-22,共6页
Journal of Suzhou Institute of Urban Construction and Environmental Protection
基金
院科研基金
关键词
生物传感器
过氧化物酶
电化学聚合
biosensor
horseradish peroxidase
N,N-diamethylaniline
electrochemical polymerization
