摘要
采用液质联用法测定血浆中阿德福韦药物浓度,并优化阿德福韦耐药基因检测方法。药物浓度测定:0.2 mL血浆中加入两倍体积的蛋白沉淀剂甲醇,高速离心后上清液吹干并用流动相甲醇-水(20︰80)溶解,应用电喷雾离子源的质谱检测;用套式PCR快速检测病人阿德福韦耐药突变。两种方法适用于临床样本检测,并可结合用于临床个体化治疗的研究。
To develop a rapid and easy to operating method for adefovir in plasma,and to optimize the method on identification of resistance genes,then to apply both methods on patients.Determination of drug concentration： The plasma samples were precipitated by methanol and centrifugated,the supernatants were volatilized by nitrogen and dissolved by mobile phase of methanol-water（20︰80）.Electrospray ionization source was applied and operated in the positive ion mode.Adefovir resisitance mutations in patients were detected by nested PCR rapidly.The proposed method showed good linearity,repeatability and recovery,and with the method on drug resistance genes it can be applied to the study of individualized therapy.
出处
《药学与临床研究》
2011年第1期18-21,共4页
Pharmaceutical and Clinical Research
关键词
阿德福韦
液质联用
耐药突变
个体化治疗
Adefovir
LC-MS/MS
Resisitance mutations
Individualized therapy
