粗叶悬钩子总生物碱对大鼠脂肪肝影响的脂肪染色图像分析

Image analysis on fat staining of fatty liver of rats with total alkaloids of Rubus Alceaefolius Poir

目的:探讨粗叶悬钩子(RAP)总生物碱对大鼠脂肪肝模型的影响。方法:以四氯化碳(CCl4)及高脂饮食造脂肪肝大鼠模型。将60只SD大鼠随机分为6组,即正常组,脂肪肝模型组,RAP总生物碱低剂量组、中剂量组、高剂量组,易善复对照组。观察各组大鼠经各种条件干预后肝脏冰冻切片的脂质变化,分别进行测量并定量分析脂肪染色阳性面积、灰度、平均光密度、积分光密度等。结果:RAP总生物碱各剂量组及易善复组均比模型组大鼠肝脏脂滴聚集变化轻,主要的测量指标平均灰度、平均光密度、积分光密度在高剂量组与模型组比较,差异具有显著性意义(P<0.05)。结论:高剂量RAP总生物碱能减轻大鼠脂肪肝模型的脂滴聚集,改善脂肪肝的病变。

Objective： To explore the effect on the fatty liver rats with the total alkaloids of Rubus Alceaefolius Poir（RAP）.Methods：The 60 rats induced by CCl4 and treated by high fat diet.Then the rats divided six groups randomly： normal group,fatty liver model group,control by RAP low dose group,RAP medium dose group,RAP high dose group,Yishanfu group.The frozen sections were fat stained by Sudan IV,then observed the pathologic change in the treated groups,and quantitative analysis of the frozen section by positive areas,gray level,average optical density,integral optical density.Results： The treated groups by polysaccharides of RAP root and Yishanfu reduced and the pathologic change in the fatty liver rats,and the RAP high dose group reduced the gray level,average optical density and the integral optical density best,the difference was significant（P0.05）.Conclusion： The RAP in high dose group can reduce the adipopexis in the fatty liver rats,and improve the pathological changes of fatty liver.