玉米自交系成熟胚愈伤组织诱导和再生体系研究

Research on Callus Induction and Regeneration from Mature Embryos of Maize

[目的]研究玉米成熟胚组织培养条件和植株再生频率的影响因素，建立玉米成熟胚高效再生体系。[方法]以玉米成熟胚为材料，通过相关影响因子对成熟胚外殖体愈伤组织诱导、继代及分化培养影响的研究。[结果]2，4-D浓度在1．0～2．0mg／L，NAA浓度为0．5—0．6mg／L明显促进成熟胚愈伤组织生长；6-BA浓度为0．15mg／L时适合愈伤组织的继代培养；新自523在三个自交系中愈伤生长质量较好，并且容易产生胚性愈伤，早21愈伤组织较少，在0．5mg／LNAA、1．5mg／L6-BA、100mg／LAgNO3浓度下旱21分化能力和新自523相当，且两者都容易生根，再生苗频率达到30％～40％，B73分化和生根能力较弱。[结论]利用玉米成熟胚诱导产生愈伤、分化并产生再生植株是继幼胚再生体系有利的补充。

[ Objective] The purpose of this research was to study the tissue culture conditions of corn matured embryo and affecting factors of plant regeneration frequency to establish high - performance regeneration system of corn matured embryo. [ Method ] The effects of correlation affecting factors on the callus induction of matured embryo explant,successive and differentiation cuhiveation were studied by use of corn matured embryo as test materials. [Result]2.4- D concentrations should be in 1.0- 2.0 mg/L between olumes concentration of 0.4- 0.5 mg/L range significantly promote the radicle callus to grow. 6 - BA concentration 0.1 - 0.02 mg/L were suitable for callus culture. The growth quality of callus of Xinzi 523 were the best. in three inbreds stirains. And it is easy to produce sexual embryo callus, but there were just few callus in Han 21. The differentiation capacity of Han21 and Xinzi 523 were identical with each other, under 0.5 mg/L NAA, 1.5 mg/L 6 - BA, 100 mg/L AgNO3, and both are easier to take root, regeneration seedling frequency reaches 30 % - 40 %, however the differentiation rate and rooting ability of B73 are relatively weak. [ Conclusion ] The callus produced by the induction of corn matured embryo, differentiation and produced explants were the favorable supplement for young embryo regeneration system.