青钱柳种质资源多样性SRAP初步分析

Preliminary Study on the Genetic Diversity of Germplasm for Cyclocarya paliurus Revealed by SRAP Markers

为了利用分子标记方法评价青钱柳种质资源的遗传多样性,本文对青钱柳DNA的提取、SRAP扩增体系重要参数进行了优化,运用优化体系筛选多态性引物,并用1对引物对青钱柳9个种源进行了遗传多样性初步分析。研究结果建立了适于青钱柳SRAP的扩增体系;从110对SRAP引物中筛选出了13对多态性引物,运用1对引物组合Me7+Em2扩增获得21个多态性位点,多态率达100%。遗传多样性分析表明有效等位基因数(Ne)为1.3429,平均Shannon's信息指数(I)为0.3687,Nei's基因多样性指数(H)为0.2267,种源的遗传分化指数Gst为0.1983;聚类分析结果表明9个种源在遗传距离0.100处聚为3类,聚类结果和地理距离之间呈现较高的相关性。本文的研究结果表明所建立的青钱柳种质资源库具有广泛的遗传多样性,为进一步的开发利用提供了条件。

In order to assess the genetic diversity of germplasm for Cyclocarya paliurus by molecular markers, DNA extraction method and key parameters of SRAP reaction system were optimized; polymorphic primers were screened with the optimal system, and preliminary analysis of genetic diversity of 9 provenances by using a pair of primers was operated in this paper. The results showed that a SRAP reaction system for Cyclocarya paliurus was established through optimal design. 13 pairs of polymorphic primers were screened from 110 pairs of SRAP markers and 21 bands were amplified using primer pair Me7＋Em2 with the polymorphic rate of 100%. Moreover, genetic diversity analysis showed that the mean Ne value of 9 provenances was 1.342 9; the mean I value, the mean H value and provenance Gst was 0.368 7, 0.226 7 and 0.198 3, respectively. The UPGMA clustering analysis displayed that 9 provenances could be divided into 3 groups at the genetic distance of 0.100, which indicated a higher correlation between the genetic distance and geographic distribution among provenances. The results of this reseach showed that germplasm of Cyclocarya paliurus we collected had widely genetic diversity, and would provide a better basis of exploitation.