摘要
本文根据在GenBank中已登录的葡萄、玉米、水稻、草莓、拟南芥和苹果等植物的类黄酮-3-O-葡萄糖基转移酶基因的核苷酸序列和氨基酸序列,应用生物信息学软件预测了其理化性质、疏水性/亲水性、导肽、跨膜结构、卷曲螺旋结构、二级结构、功能结构域及高级结构,并构建了类黄酮-3-O-葡萄糖基转移酶蛋白家族的系统进化树。结果表明这几种植物的UFGT基因核苷酸序列除葡萄存在2个外显子外,其它植物均存在1个外显子。含量最丰富的氨基酸是Leu、Ala、Gly、Val和Ser等,除葡萄、苹果UFGT属于不稳定类蛋白外,其余均属于稳定类蛋白。进一步研究分析表明,这几种植物UFGT蛋白存在明显的疏水区和亲水区、信号肽、跨膜结构以及卷曲螺旋。二级结构组成上比例相似,并且都由α-螺旋、延伸链和无规则卷曲所组成。它们都存在UDPGT、COG1819和MGT等保守域。除了拟南芥外,其它几种植物都能够通过同源建模建立UFGT蛋白的三维结构。进化分析表明,把它们的UFGT基因分成5个类群,其中3个大类群,另外陆地棉和野芭蕉的UFGT基因分别单独成一类。本工作将为深入研究该蛋白在植物花、果实和叶片等器官颜色变化中发挥的功能,开展生物大分子结构模拟以及药物设计提供抛砖引玉的作用。
In this paper, the nucleic acid sequences and amino acid sequences of flavonoid-3-O-glucosyltransferas from six species such as Vitis vinifera, Zea mays, Oryza sativa, Fragaria×ananassa, Arabidopsis thaliana and Malus domestica, were analyzed by bioinformatics tools. Several parameters of these sequences logined in GeneBank, including sequences composition, physicochemical property, leader peptide, topological structure of transmembrane regions, hydrophobicity or hydrophilicity, secondary structures, functional domains and protein structures, were predicted. Phylogenetic tree was reconstructed for the flavonoid-3-O-glucosy-ltransferase protein family. UFGT genes contain only one exon in most of these plants, but two exons in V. vinifera. Abundant with Leu, Ala, Gly, Val and Ser amino acids, most of these UFGT proteins are structural stable except for those from V. vinifera and apple. In all these UFGT protein, evident hydrophobic or hydrophilic domain, signal peptide, transmembrane region and coiled-coil domain were predicted; and similar ratio in secondary structure composition made from α-helixes, sheets and random coils were detected. All these proteins have UDPGT, COG1819, MGT conserved domains. The 3-dimensional structures were constructed through rough homology modeling method successfully for most of these proteins but not for the one from Arabidopsis. In evolutionary analysis all these UFGT proteins were divided into five groups, among which were three major ones and two single one that from Gossypium hirsutum L. and Camptotheca acuminate respectively. This work would provide a basis for the function determination of this protein in color variance of different plant organs, such as flowers, fruits and leaves, and develop biological macromolecule structual simulation and molecular drug design.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2011年第1期92-102,共11页
Genomics and Applied Biology
基金
西南林业大学科研启动基金项目,西南林业大学云南省重点学科森林培育学(XKZ200906),西南林业大学重点建设学科林木遗传育种(XKX200904)共同资助
国家林业局西南地区生物多样性保育重点实验室开放基金项目(BC2010F08)