体外培养人胎肾上腺髓质家兔脑内移植的研究

Graft of Human Fetal Adrenal Medullary Tissue Cultured in Vitro to the Subcortex of the Adult rabbit

培养妊娠16、20和24周人胎肾上腺髓质组织2周,观察组织细胞形态和功能变化。结果提示,妊娠24周人胎肾上腺髓质组织功能较妊娠16和20周者为好。培养8天的妊娠24周人胎肾上腺髓质组织移植至24只成年家兔脑内。术后1周检查4只动物,3只移植物存活良好,1只部分存活。1个月时检查10只动物,6只移植物存活良好,1只部分存活,3只移植物消失。3个月时检查10只动物,5只移植物存活良好,1只部分存活,4只移植物消失。移植物存活良好者具有儿茶酚胺(CA)合成分泌功能。移植物部分存活及消失者可能与移植过程损伤和排异反应有关。

Human fetal adrenal medullary tissue removed from the fetus donor aborted atthe age of 16,20 and 24 week′s gestation was cultured for two weeks The morphologyand function of the tissue and cells were observed.The results suggest that the adrenal medullary tissue cultured from the fetus donor aborted at 24 week′s gestationhas high functional secretory of DA.The tissue cultured was transplanted to thesubcortex in twenty four adult rabbits.This is the first demonstration that the humanfetal adrenal medullary tissue cultured in vitro can survive when it was implantedinto the subcortex of adult rabbits.