用中和试验证明空肠弯曲菌产生霍乱样肠毒素

Identification of Cholera-like Enterotoxin Produced by Campylobacter Jejuni with Neutralization Test

本实验应用霍乱肠毒素(CT)和大肠杆菌不耐热肠毒素(LT)抗血清.对3株空肠弯曲菌的粗制肠毒素进行中和试验,观察其是否能中和这些肠毒素使CHO 细胞伸长的活性,以评价空肠弯曲菌肠毒素(CJT)和霍乱肠毒素之间的关系。结果表明,这些肠毒素促使CHO 细胞由圆形变为梭形的活性,可因CT 或LT 抗血清的预处理而消失,从而进一步证明空肠弯曲菌中某些菌株可产生霍乱样肠毒素。

In this paper,the relationship between CJT and CT is evaluated by neutraliza-tion test.The results show that the cytotonic activity of crude enterotoxin producedby 3 strains of campylobacter jejuni to CHO cells is abolished by preincubation withCT or LT antiserum.This experiment has further proved that some of campylobacterjejuni can produce Cholera-like enterotoxin.