摘要
本文采用间接免疫荧光法,检测单抗C_3H_+6和C_4H_8与33例不同类型白血病患者的外周血细胞的特异性结合反应,发现C_3H_6、C_4H_8抗原主要表达在急性髓性白血病(AML)细胞上。该抗原在其他类型白血病中的阳性表达率低于20%。用这种方法检测各亚型,M_5型检出率最高,且荧光强度最好,其次为M_4、M_1型。此结果提示单抗C_3H_6和C_4H_8对于临床鉴别急淋与急非淋白血病,以及区分AML 各亚型,有一定实用价值,结合其他检测手段,能使白血病的FAB 分型更为准确。
A specifical reaction between peripheral blood cells of 33 patients with leukemiaand C_3H_6,C_4H_8 monoclonal antibodies was determined with indirect immunoflore-scence method.C_3H_6,C_4H_8 antigens were mainly presented in the cells of acute mye-logenous leukemia(AML).In other types of leukemia,the rate of positive presenceof the antigen was under 20 percent.In subtype of AML,the positivity rate of M_5and the intensity of fluorescence were the highest,while that were the next highest intype of M_4 and M_1.The results show that C_3H_6 and C_4H_8 monoclonal antibodies areof great worth in distinguishing all such kinds of monoclonal antibodies with AMLand the subtype of AML.
出处
《苏州医学院学报》
1990年第3期210-211,220,共3页
Acta Academiae Medicinae Suzhou
关键词
白血病
单克隆抗体
leukemia
monoclonal antibody