四氯化碳法兔肝硬化模型的建立

Establishment of Liver Cirrhosis in Rabbit by subcutaneous injection of carbon tetrachloride

目的探讨通过皮下注射四氯化碳(carbon tetrachloride,CCl4)-橄榄油溶液制备兔肝硬化模型的可行性。方法体重在2.0-2.5 kg的新西兰大白兔42只,其中实验组36只,分成三组(Ⅰ、Ⅱ、Ⅲ),每组12只,前两周均每周两次皮下注射50%的CCl4-橄榄油溶液0.3 ml/kg,第三周起Ⅰ组每周两次皮下注射50%的CCl4-橄榄油溶液1.5ml/kg;Ⅱ组每周两次皮下注射50%的CCl4-橄榄油溶液0.9 ml/kg;Ⅲ组每周两次皮下注射50%的CCl4-橄榄油溶液0.4 ml/kg,对照组6只,分成三组,每组2只,分别与实验组相对应注射等量的橄榄油,实验组与对照组均于第12周末处死并取肝组织做病理。结果三个实验组均造成肝硬化模型,实验过程中兔子死亡率分别为67%、50%、8.3%。结论皮下注射四氯化碳-橄榄油溶液制备兔肝硬化模型可行,且Ⅲ组造模成功率高,适用于临床研究。

Objective To evaluate the feasibility of rabbit model of liver cirrhosis established by subcutaneous injection of carbon tetrachloride-olive oil solution.Methods 42 body weight during 2.0-2.5 kg New Zealand white rabbits,36 in experimental group were divided into three groups（Ⅰ,Ⅱ,Ⅲ）,12 in each group,the first two weeks was injected subcutaneously by 0.3 ml/kg 50% carbon tetrachloride-olive oil solution twice-weekly,from the third week Ⅰ,Ⅱ,Ⅲ was injected subcutaneously by 1.5 ml/kg,0.9 ml/kg,0.4 ml/kg respectively 50% carbon tetrachloride-olive oil solution twice-weekly;6 in control group were divided into three groups,2 in each group corresponding to the experimental group,was injected with the same amount of olive oil,all rabbits were sacrificed at 12 weeks and obtained liver tissues for pathology.Results Three experimental groups turned into liver cirrhosis models,rabbit mortality rate during the experiment was 67%,50%,8.3% respectively.Conclusion The establishment of liver cirrhosis in rabbit by subcutaneous injection of carbon tetrachloride-olive oil solution was feasible,and group Ⅲ had a high success rate and fitted for clinical research.