蛋白质相互作用的研究方法

Research techniques for protein-protein interactions

通过研究蛋白质-蛋白质的相互作用,能够更好地注释蛋白质功能,解码生命现象。蛋白质相互作用研究方法的快速发展与不断完善为蛋白质间相互作用的研究奠定了坚实基础。本文对当前研究蛋白质相互作用的主要技术方法,包括酵母双杂交技术,GSTpull-down技术,免疫共沉淀技术、串联亲和纯化技术等多种研究方法进行综述。

Protein function can be identified and some phenomena of life can be elucidated by the study of protein-protein interaction.The techniques on protein-protein interactions have been developped and improved.The subject of this paper is to review the classICaL and especially recent methods of study protein-protein interactions such as Yeast Two-Hybrid System,GST pull-down,co-immunoprecipitation,tandem affinity purification.