50种木材提取物清除自由基活性初探

Primary Studies on Free Radical Scavenging Activities of Extracts from 50 Kinds of Woods

用二苯基苦基苯肼自由基(DPPH.)酶标仪法,对亚热带常见的50种木材甲醇提取物的自由基清除活性进行了比较,发现不同木材提取物的自由基清除活性差别很大,最大相差15.48倍,其中刺槐、桑树、李树、桃树和杉木的木材提取物具有较强的清除自由基活性,它们单位质量干样的半数清除质量浓度(IC50)分别为:0.52、0.64、0.66、0.67、1.00 g/L。活性较弱的主要有银杏、朴树、广玉兰和加杨等。方差分析结果表明:耐腐性"强"和"弱"的木材提取物的清除自由基活性差异达到显著性水平。

l, 1-Dipheny-2-picrylhydrazine free radical（ DPPH. ）-microplate assay was used to determine the free radical scavenging activity of methanolic extracts from 50 kinds of woods. Results revealed that these woods had very different radical scavenging activity. The maximum activity was 15.48 times of the minimum. Among the 50 kinds of woods, Robinia pseudoacacia, Morus alba, Prunus salicina, Amygdalus persica and Cunninghamia lanceolata had strong radical scavenging activity. Their half-inhibi- tory＇-concentrations（IC50） of dry samples were 0.52, 0.64, 0.66, 0.67 and 1.00 g/L respectively. Among the tested woods, Ginkgo biloba, Celtis sinensis, Magnolia grandiflora and Populus canadensis had the lowest radical scavenging activity. Results of variance analysis showed that the difference of radical scavenging activity between the woods possessing strong and weak decay resistance was significant.