蔗糖和聚乙二醇对黄金梨花粉离体萌发生长的影响

Effects of Sucrose and PEG-4000 on Pollen Germnation and Pollen Tube Growth of Pyrus pyrifolia cv.Whangkeumbae in Vitro

在含有不同浓度蔗糖和聚乙二醇-4000(PEG-4000)的培养基上对黄金梨[Pyrus pyrifolia(Burm.F.)Nakai cv.Whangkeumbae]的花粉离体培养和花粉管生长情况进行了研究。结果表明,当蔗糖和PEG-4000的浓度逐渐升高时,花粉萌发率和花粉管长度都逐渐增加,但浓度过高时表现出一定的抑制作用。当蔗糖浓度为100 g/L时,花粉萌发率达41.3%,花粉管长度达到693μm;当PEG-4000浓度为15 g/L时,花粉萌发率为39.9%,花粉管长度为675μm。最适合黄金梨花粉离体培养的培养基组成为H3BO4 50mg/L+MgSO4 10 mg/L+蔗糖100 g/L+PEG-4000 15 g/L。

The effects of different concentrations of sucrose and PEG-4000 on pollen germination and pollen tube growth of Pyrus pyrifolia（Burm.F.）Nakai cv.Whangkeumbae were studied.The results showed that appropriate amount of sucrose and PEG-4000 was conductive to the pollen germination and the pollen tube growth.But when the concentration was too high,it inhibited pollen germination and pollen tube growth.When the sucrose concentration was 100 g/L,the pollen germination rate and pollen tube length reached 41.3%and 693μm respectively.When the PEG-4000 concentration was 15 g/L,the pollen germination rate and pollen tube length reached 39.9%and 675μm respectively.The concentration of 50 mg/L H3BO4,10 mg/L MgSO4,100 g/L sucrose and 15 g/L PEG-4000 was suitable to pollen germination and pollen tube growth.