摘要
蛋白粉经萃取,过PCX柱提纯后,采用Symmetry-C18柱,以庚烷磺酸钠缓冲溶液-甲醇(95∶5,V/V)为流动相,流速1.0 mL/min,测定蛋白粉中三聚氰胺的含量。该方法在1～100.0 mg/L范围内,相对标准偏差小于0.04%,标准曲线呈良好的线性关系(R2=0.999 1),最低检出限为1.0 mg/L,样品加标回收率为98.7%～104.0%,相对标准偏差小于0.4%,该方法能快速有效地测定蛋白粉中三聚氰胺的含量。
A HPLC method for the determination of melamine in protein powder was developed.Melamine in sample was extracted and purified by PCX column,then separated in Symmetry-C18 column with a mobile phase consisting of sodium heptanesulfonate and methanol（95：5,V/V）at 1.0 mL/min.The method showed a good linear relationship（r=0.999 1）for melamine of 1～100.0 mg/L with relative standard deviation was less than 0.4%,the detection limit was 1.0 mg/L,the recoveries ranged from 98.7%to 104%with relative standard deviations less than 0.04%.The method was a fast and accurate one for detecting melamine in protein powder.
出处
《湖北农业科学》
北大核心
2011年第2期378-380,共3页
Hubei Agricultural Sciences
基金
南通农业职业技术学院"青蓝工程"
